A direct link between core histone acetylation and transcriptionally active chromatin - PubMed (original) (raw)
A direct link between core histone acetylation and transcriptionally active chromatin
T R Hebbes et al. EMBO J. 1988 May.
Abstract
An antiserum raised against chemically acetylated histone H4 was found to recognize the epitope epsilon-N-acetyl lysine. Affinity-purified antibodies were used to fractionate oligo- and mononucleosomal chromatin fragments from the nuclei of 15-day chicken embryo erythrocytes. Antibody-bound chromatin was found to contain elevated levels of acetylated core histones. On probing with sequences of alpha D globin, an actively transcribed gene, the antibody-bound chromatin was 15- to 30-fold enriched relative to the input chromatin. Using ovalbumin sequences as a probe, no enrichment was observed. The results demonstrate directly that transcriptionally active genes carry acetylated core histones.
Similar articles
- Histone acetylation and globin gene switching.
Hebbes TR, Thorne AW, Clayton AL, Crane-Robinson C. Hebbes TR, et al. Nucleic Acids Res. 1992 Mar 11;20(5):1017-22. doi: 10.1093/nar/20.5.1017. Nucleic Acids Res. 1992. PMID: 1549462 Free PMC article. - Chromosomal mapping of core histone acetylation by immunoselection.
Crane-Robinson C, Hebbes TR, Clayton AL, Thorne AW. Crane-Robinson C, et al. Methods. 1997 May;12(1):48-56. doi: 10.1006/meth.1997.0446. Methods. 1997. PMID: 9169194 - Acetylation of histone H2B mirrors that of H4 and H3 at the chicken beta-globin locus but not at housekeeping genes.
Myers FA, Chong W, Evans DR, Thorne AW, Crane-Robinson C. Myers FA, et al. J Biol Chem. 2003 Sep 19;278(38):36315-22. doi: 10.1074/jbc.M305822200. Epub 2003 Jul 14. J Biol Chem. 2003. PMID: 12865423 - Nuclear matrix, dynamic histone acetylation and transcriptionally active chromatin.
Davie JR. Davie JR. Mol Biol Rep. 1997 Aug;24(3):197-207. doi: 10.1023/a:1006811817247. Mol Biol Rep. 1997. PMID: 9291093 Review. - Chicken Erythrocyte: Epigenomic Regulation of Gene Activity.
Beacon TH, Davie JR. Beacon TH, et al. Int J Mol Sci. 2023 May 5;24(9):8287. doi: 10.3390/ijms24098287. Int J Mol Sci. 2023. PMID: 37175991 Free PMC article. Review.
Cited by
- Native ChIP: Studying the Genome-Wide Distribution of Histone Modifications in Cells and Tissue.
Nitsch S, Schneider R. Nitsch S, et al. Methods Mol Biol. 2024;2846:1-16. doi: 10.1007/978-1-0716-4071-5_1. Methods Mol Biol. 2024. PMID: 39141226 - Emerging toolkits for decoding the co-occurrence of modified histones and chromatin proteins.
Pepin AS, Schneider R. Pepin AS, et al. EMBO Rep. 2024 Aug;25(8):3202-3220. doi: 10.1038/s44319-024-00199-2. Epub 2024 Aug 2. EMBO Rep. 2024. PMID: 39095610 Free PMC article. Review. - The role of HDAC3 in inflammation: mechanisms and therapeutic implications.
Watson N, Kuppuswamy S, Ledford WL, Sukumari-Ramesh S. Watson N, et al. Front Immunol. 2024 Jul 10;15:1419685. doi: 10.3389/fimmu.2024.1419685. eCollection 2024. Front Immunol. 2024. PMID: 39050859 Free PMC article. Review. - HBO1, a MYSTerious KAT and its links to cancer.
Yokoyama A, Niida H, Kutateladze TG, Côté J. Yokoyama A, et al. Biochim Biophys Acta Gene Regul Mech. 2024 Sep;1867(3):195045. doi: 10.1016/j.bbagrm.2024.195045. Epub 2024 Jun 6. Biochim Biophys Acta Gene Regul Mech. 2024. PMID: 38851533 Review. - Unraveling the Progression of Colon Cancer Pathogenesis Through Epigenetic Alterations and Genetic Pathways.
Abolghasemi Fard A, Mahmoodzadeh A. Abolghasemi Fard A, et al. Cureus. 2024 May 2;16(5):e59503. doi: 10.7759/cureus.59503. eCollection 2024 May. Cureus. 2024. PMID: 38826873 Free PMC article. Review.
References
- Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12 - PubMed
- Proc Natl Acad Sci U S A. 1964 May;51:786-94 - PubMed
- J Biol Chem. 1970 Mar 25;245(6):1261-6 - PubMed
- J Cell Biol. 1973 Jun;57(3):773-81 - PubMed
- J Biol Chem. 1974 Jun 25;249(12):3960-72 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources