A direct link between core histone acetylation and transcriptionally active chromatin - PubMed (original) (raw)
A direct link between core histone acetylation and transcriptionally active chromatin
T R Hebbes et al. EMBO J. 1988 May.
Abstract
An antiserum raised against chemically acetylated histone H4 was found to recognize the epitope epsilon-N-acetyl lysine. Affinity-purified antibodies were used to fractionate oligo- and mononucleosomal chromatin fragments from the nuclei of 15-day chicken embryo erythrocytes. Antibody-bound chromatin was found to contain elevated levels of acetylated core histones. On probing with sequences of alpha D globin, an actively transcribed gene, the antibody-bound chromatin was 15- to 30-fold enriched relative to the input chromatin. Using ovalbumin sequences as a probe, no enrichment was observed. The results demonstrate directly that transcriptionally active genes carry acetylated core histones.
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References
- Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12 - PubMed
- Proc Natl Acad Sci U S A. 1964 May;51:786-94 - PubMed
- J Biol Chem. 1970 Mar 25;245(6):1261-6 - PubMed
- J Cell Biol. 1973 Jun;57(3):773-81 - PubMed
- J Biol Chem. 1974 Jun 25;249(12):3960-72 - PubMed
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