Effects of Dietary Valine Levels on Production Performance, Egg Quality, Antioxidant Capacity, Immunity, and Intestinal Amino Acid Absorption of Laying Hens during the Peak Lay Period - PubMed (original) (raw)

Effects of Dietary Valine Levels on Production Performance, Egg Quality, Antioxidant Capacity, Immunity, and Intestinal Amino Acid Absorption of Laying Hens during the Peak Lay Period

Huafeng Jian et al. Animals (Basel). 2021.

Abstract

The present study aimed to assess the impact of dietary valine levels on layer production performance, egg quality, immunity, and intestinal amino acid absorption of laying hens during the peak lay period. For this purpose, a total of 960 33-week-old Fengda No.1 laying hens were randomly divided into five experimental groups and fed with valine at the following different levels in a feeding trial that lasted 8 weeks: 0.59, 0.64, 0.69, 0.74, and 0.79%, respectively. Productive performances were recorded throughout the whole rearing cycle and the egg quality, serum indexes, and small intestine transporters expression were assessed at the end of the experiment after slaughter (41 weeks) on 12 hens per group. Statistical analysis was conducted by one-way ANOVA followed by LSD multiple comparison tests with SPSS 20.0 (SPSS, Chicago, IL, USA). The linear and quadratic effects were tested by SPSS 20.0. Egg mass, laying rate, broken egg rate, and feed conversion ratio were significantly improved with increasing dietary valine levels. However, the egg weight, eggshell thickness, albumen height, Haugh unit, and egg yolk color were significantly decreased with increasing dietary valine levels. Serum catalase (CAT), immunoglobulin A (IgA) and IgM levels, and malondialdehyde (MDA) levels were negative responses to valine-treated laying hens. Dietary supplemented valine enhanced the trypsin activity of duodenum chime and promoted the mRNA expression levels of ATB0,+, and LAT4 in the jejunum and corresponding serum free Ile, Lys, Phe, Val, and Tyr level. However, valine treatment significantly downregulated the mRNA expression levels of PePT1, B0AT1, LAT1, and SNAT2 in the small intestines and corresponding serum free Arg, His, Met, Thr, Ala, Asp, Glu, Gly, and Ser level. Our results suggest that 0.79% valine dietary supplementation can improve production performance by promoting amino acid nutrient uptake and utilization, and suggest a supplement of 0.79% valine to diet.

Keywords: Fengda No.1 laying hens; antioxidant enzymes; neutral amino acid transporters; serum free amino acids; valine.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Figure 1

Figure 1

Effects of dietary valine levels on serum immune indices of laying hens. Values are represented as the mean and SEM (n = 8). Means with different superscript letters (a–c) within a column differ significantly (p < 0.05). IgA, Immunoglobulin A; IgM, Immunoglobulin M; IgG, Immunoglobulin G.

Figure 2

Figure 2

Effects of dietary valine levels on activities of duodenum digestive enzymes of laying hens. Values are represented as the mean and SEM (n = 8). Means with different superscript letters (a, b) within a column differ significantly (p < 0.05).

Figure 3

Figure 3

Effects of dietary valine levels on mRNA expression levels of PepT1, B0AT1, SNAT2, ATB0,+, LAT1, and LAT4 in the duodenum of laying hens. Values are represented as the means and SEM (n = 6–8). Means with different superscript letters (a–c) within a column differ significantly (p < 0.05).

Figure 4

Figure 4

Effects of dietary valine levels on mRNA expression levels of PepT1, B0AT1, SNAT2, ATB0,+, LAT1, and LAT4 in jejunum of laying hens. Values are represented as the mean and SEM (n = 6–8). Means with different superscript letters (a–c) within a column differ significantly (p < 0.05).

Figure 5

Figure 5

Effects of dietary valine levels on mRNA expression levels of PepT1, B0AT1, SNAT2, ATB0,+, LAT1, and LAT4 in ileum of laying hens. Values are represented as the mean and SEM (n = 6–8). Means with different superscript letters (a–c) within a column differ significantly (p < 0.05).

References

    1. Abbate J.M., Macrì F., Capparucci F., Iaria C., Briguglio G., Cicero L., Salvo A., Arfuso F., Ieni A., Piccione G., et al. Administration of Protein Hydrolysates from Anchovy (Engraulis encrasicolus) Waste for Twelve Weeks Decreases Metabolic Dysfunction-Associated Fatty Liver Disease Severity in ApoE−/− Mice. Animals. 2020;10:2303. doi: 10.3390/ani10122303. - DOI - PMC - PubMed
    1. Avondo M., Pagano R.I., Guastella A.M., Criscione A., Di Gloria M., Valenti B., Piccione G., Pennisi P. Diet Selection and Milk Production and Composition in Girgentana Goats with Different Alpha s1-casein Genotype. J. Dairy Res. 2009;76:202–209. doi: 10.1017/S0022029909003914. - DOI - PubMed
    1. Monteverde V., Congiu F., Vazzana I., Dara S., Di Pietro S., Piccione G. Serum Lipid Profile Modification Related to Polyunsaturated Fatty Acid Supplementation in Thoroughbred Horses. J. Appl. Anim. Res. 2017;45:615–618. doi: 10.1080/09712119.2016.1251439. - DOI
    1. Armato L., Gianesella M., Morgante M., Fiore E., Rizzo M., Giudice E., Piccione G. Rumen Volatile Fatty Acids × Dietary Supplementation with Live Yeast and Yeast Cell Wall in Feedlot Beef Cattle. Acta Agric. Scand. Sect. A Anim. Sci. 2016;66:119–124. doi: 10.1080/09064702.2016.1272628. - DOI
    1. Brosnan J.T., Brosnan M.E. Branched-Chain Amino Acids: Enzyme and Substrate Regulation. J. Nutr. 2006;136:207S–211S. doi: 10.1093/jn/136.1.207S. - DOI - PubMed

LinkOut - more resources