Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg - PubMed (original) (raw)

Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg

V E Worrell et al. J Bacteriol. 1988 Feb.

Abstract

A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions.

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