Cytogenetic evidence of gene amplification as a mechanism for tumor cell invasion - PubMed (original) (raw)
Cytogenetic evidence of gene amplification as a mechanism for tumor cell invasion
S J Bevacqua et al. Somat Cell Mol Genet. 1988 Jan.
Abstract
In order to study the process by which human melanoma cells achieve invasion of basement membranes, a modification of the Membrane Invasion Culture System was developed to allow the in vitro collection of human melanoma cell populations that had invaded acellular human amniotic membranes. A significant increase in the number of double-minute chromosomes (DMs) was observed in metaphase nuclei of A375P human melanoma cells which had passed through two amniotic membranes (A375P-2) over that of control cells. Eighteen percent of the first monolayer of A375P-2 cells contained 1-89 DMs/cell, whereas 3-8.3% of the control A375P cells contained 1-10 DMs/cell. There was a rapid loss of DMs in A375P-2 cells as a function of passage number. After 25 days in tissue culture, the incidence of DMs had essentially dropped below the control range. These data indicate that an unstable gene amplification event may be part of the process by which melanoma cells execute invasion through basement membranes.
Similar articles
- A comparison of levels of intrinsic single strand breaks/alkali labile sites associated with human melanoma cell invasion.
Meade-Tollin LC, Pipes BL, Anderson SJ, Seftor EA, Hendrix MJ. Meade-Tollin LC, et al. Cancer Lett. 1990 Aug;53(1):45-54. doi: 10.1016/0304-3835(90)90009-m. Cancer Lett. 1990. PMID: 2397481 - Cloning efficiency of human melanoma cells is modulated after invasion through a reconstituted basement membrane.
Yohem KH, Seftor EA, Meyskens FL Jr, Hendrix MJ. Yohem KH, et al. Cancer Lett. 1989 May;45(2):135-43. doi: 10.1016/0304-3835(89)90148-1. Cancer Lett. 1989. PMID: 2731157 - Tumorigenicity of ten karyotypically distinct cell types present in the human melanoma cell line MeWo-A.
Hough MR, White BN, Holden JJ. Hough MR, et al. Cancer Genet Cytogenet. 1988 May;32(1):117-28. doi: 10.1016/0165-4608(88)90318-4. Cancer Genet Cytogenet. 1988. PMID: 3162702 - Double minute chromosomes and homogeneously staining regions in tumors taken directly from patients versus in human tumor cell lines.
Benner SE, Wahl GM, Von Hoff DD. Benner SE, et al. Anticancer Drugs. 1991 Feb;2(1):11-25. doi: 10.1097/00001813-199102000-00002. Anticancer Drugs. 1991. PMID: 1720337 Review. - [Use of reconstituted basal membranes for the study of invasion of human tumor cells: current status and future prospects].
Visconti P, Percario M, Melchiori A, Aresu O, Fassina G, Corradino P, Carlone S, Brega I, Vigani A, Pichi E, et al. Visconti P, et al. Boll Soc Ital Biol Sper. 1990 Apr;66(4):365-72. Boll Soc Ital Biol Sper. 1990. PMID: 2202333 Review. Italian.
Cited by
- Progression: the terminal stage in carcinogenesis.
Pitot HC. Pitot HC. Jpn J Cancer Res. 1989 Jul;80(7):599-607. doi: 10.1111/j.1349-7006.1989.tb01683.x. Jpn J Cancer Res. 1989. PMID: 2507482 Free PMC article. Review. No abstract available. - Tumor progression: potential role of unstable genomic changes.
Hill RP. Hill RP. Cancer Metastasis Rev. 1990 Sep;9(2):137-47. doi: 10.1007/BF00046340. Cancer Metastasis Rev. 1990. PMID: 2253313 Review. - Hypoxia induces DNA overreplication and enhances metastatic potential of murine tumor cells.
Young SD, Marshall RS, Hill RP. Young SD, et al. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9533-7. doi: 10.1073/pnas.85.24.9533. Proc Natl Acad Sci U S A. 1988. PMID: 3200838 Free PMC article.