A 40-base-pair sequence in the 3' end of the beta-actin gene regulates beta-actin mRNA transcription during myogenesis - PubMed (original) (raw)
A 40-base-pair sequence in the 3' end of the beta-actin gene regulates beta-actin mRNA transcription during myogenesis
L DePonti-Zilli et al. Proc Natl Acad Sci U S A. 1988 Mar.
Abstract
In an earlier report, evidence was presented that the down-regulation of beta-actin mRNA during myogenesis was controlled by a region 3' to the promoter of the gene. In this paper we report the location of this regulatory sequence, determined by deletion analysis and the use of chimeric genes, transfected stably into the mouse myogenic cell line C2C12. The domain responsible for the reduction in beta-actin mRNA levels is at most 40 base pairs long and is located just 5' to the canonical polyadenylylation signal in the gene. Placement of this sequence in the corresponding 3' position both in the alpha-cardiac-actin gene and in the neomycin-resistance gene in pSV2-neo confers the beta-actin mRNA regulatory pattern when these constructs are stably introduced into C2C12 cells. Nuclear run-on experiments indicate that transcriptional control can account for the decrease observed in beta-actin mRNA levels during myogenesis for both the endogenous as well as the transfected beta-actin gene constructs. This 3' transcriptional control sequence is conserved in all of the vertebrate beta-actin genes sequenced and is not similar to any of the 3' processing-adenylylation or termination sequences described previously. This mode of gene regulation may reflect a more general mechanism involved in the process of gene suppression during development.
Similar articles
- The down-regulation of the chicken cytoplasmic beta actin during myogenic differentiation does not require the gene promoter but involves the 3' end of the gene.
Lohse P, Arnold HH. Lohse P, et al. Nucleic Acids Res. 1988 Apr 11;16(7):2787-803. doi: 10.1093/nar/16.7.2787. Nucleic Acids Res. 1988. PMID: 2835747 Free PMC article. - Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.
Seiler-Tuyns A, Eldridge JD, Paterson BM. Seiler-Tuyns A, et al. Proc Natl Acad Sci U S A. 1984 May;81(10):2980-4. doi: 10.1073/pnas.81.10.2980. Proc Natl Acad Sci U S A. 1984. PMID: 6328484 Free PMC article. - Regulation of chicken alpha and beta actin genes and their hybrids inserted into myogenic mouse cells.
Sharp SB, Kost TA, Hughes SH, Davidson N. Sharp SB, et al. Gene. 1989 Aug 15;80(2):293-304. doi: 10.1016/0378-1119(89)90293-x. Gene. 1989. PMID: 2573560 - Multiple 5'-flanking regions of the human alpha-skeletal actin gene synergistically modulate muscle-specific expression.
Muscat GE, Kedes L. Muscat GE, et al. Mol Cell Biol. 1987 Nov;7(11):4089-99. doi: 10.1128/mcb.7.11.4089-4099.1987. Mol Cell Biol. 1987. PMID: 2828926 Free PMC article.
Cited by
- Nucleotide- and Protein-Dependent Functions of Actg1.
Sundby LJ, Southern WM, Hawbaker KM, Trujillo JM, Perrin BJ, Ervasti JM. Sundby LJ, et al. Mol Biol Cell. 2022 Aug 1;33(9):ar77. doi: 10.1091/mbc.E22-02-0054. Epub 2022 May 20. Mol Biol Cell. 2022. PMID: 35594181 Free PMC article. - A novel actin mRNA splice variant regulates ACTG1 expression.
Drummond MC, Friderici KH. Drummond MC, et al. PLoS Genet. 2013;9(10):e1003743. doi: 10.1371/journal.pgen.1003743. Epub 2013 Oct 3. PLoS Genet. 2013. PMID: 24098136 Free PMC article. - Characterization of four heat-shock protein genes from Nile tilapia (Oreochromis niloticus) and demonstration of the inducible transcriptional activity of Hsp70 promoter.
Zhang L, Sun C, Ye X, Zou S, Lu M, Liu Z, Tian Y. Zhang L, et al. Fish Physiol Biochem. 2014 Feb;40(1):221-33. doi: 10.1007/s10695-013-9838-y. Epub 2013 Aug 4. Fish Physiol Biochem. 2014. PMID: 23912482 - Effective generation of transgenic reporter and gene trap lines of the medaka (Oryzias latipes) using the Ac/Ds transposon system.
Froschauer A, Sprott D, Gerwien F, Henker Y, Rudolph F, Pfennig F, Gutzeit HO. Froschauer A, et al. Transgenic Res. 2012 Feb;21(1):149-62. doi: 10.1007/s11248-011-9514-x. Epub 2011 Apr 30. Transgenic Res. 2012. PMID: 21533666 - Isolation of Tanichthys albonubes beta actin gene and production of transgenic Tanichthys albonubes.
Yu EM, Ye X, Wang HY, Bai JJ, Xia SL, Lao HH, Jian Q. Yu EM, et al. Fish Physiol Biochem. 2010 Jun;36(2):173-80. doi: 10.1007/s10695-008-9238-x. Epub 2008 Jun 19. Fish Physiol Biochem. 2010. PMID: 20467859
References
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
- Nucleic Acids Res. 1984 Feb 10;12(3):1687-96 - PubMed
- Nucleic Acids Res. 1983 Dec 10;11(23):8287-301 - PubMed
- Proc Natl Acad Sci U S A. 1984 May;81(10):2980-4 - PubMed
- Cell. 1985 Jun;41(2):349-59 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources