Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B - PubMed (original) (raw)

Comparative Study

Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B

O Grépinet et al. Nucleic Acids Res. 1986.

Free PMC article

Abstract

The nucleotide sequence of the CelB gene, encoding the extracellular endoglucanase B of Clostridium thermocellum, is reported. The putative start of the 1689 bp coding sequence was assigned to an ATG codon which is preceded by an AGGAGG sequence typical of ribosomal binding sites in Gram-positive bacteria. The amino-terminal end of the deduced protein sequence is similar to signal peptides described for other bacterial secretory proteins. The carboxy-terminal ends of endoglucanases A and B appear to be remarkably homologous. A striking feature of the conserved region is that both proteins contain two reiterated stretches of 23 aminoacids each, separated by 9 residues.

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References

    1. Annu Rev Genet. 1979;13:319-53 -PubMed
    1. J Biol Chem. 1984 Feb 10;259(3):1695-702 -PubMed
    1. Anal Biochem. 1983 Feb 15;129(1):216-23 -PubMed
    1. Nature. 1982 Aug 19;298(5876):760-2 -PubMed
    1. Proc Natl Acad Sci U S A. 1983 Feb;80(3):687-91 -PubMed

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