Antigenic competition at the level of peptide-Ia binding - PubMed (original) (raw)

Antigenic competition at the level of peptide-Ia binding

B P Babbitt et al. Proc Natl Acad Sci U S A. 1986 Jun.

Abstract

We examined the direct binding of a hen egg white lysozyme peptide, HEL(46-61), to membrane I-Ak (protein encoded in the A locus of the I region) molecules in the presence of detergent. A number of synthetic peptide derivatives, which did not stimulate our T-cell reactive hybridomas, competed for the binding of HEL(46-61) to I-Ak and also inhibited the functional presentation of HEL(46-61). Inhibitors included a peptide lacking a tyrosine at position 53 and a peptide corresponding to the autologous lysozyme peptide. Presentation was examined with cells or with supported planar phospholipid membranes bearing only I-Ak and HEL(46-61). Other peptides that did not compete for the binding did not inhibit functional presentation. We concluded that the binding of an immunogenic peptide to I-A is critical for presentation, that the I-A molecule does not discriminate between autologous and foreign related determinants but does recognize structurally different peptides. Our evidence suggests that our immunogenic peptide bears noncontiguous amino acids critical for contact I-A binding interspersed with amino acids critical for interaction with T cells.

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References

    1. Biochim Biophys Acta. 1973 Nov 11;328(1):20-30 - PubMed
    1. J Mol Cell Immunol. 1983;1(1):3-18 - PubMed
    1. Biochemistry. 1982 Sep 28;21(20):5020-31 - PubMed
    1. J Immunol. 1983 Feb;130(2):637-43 - PubMed
    1. J Exp Med. 1983 May 1;157(5):1618-34 - PubMed

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