Evolutionary and genetic implications of sequence variation in two nonallelic HLA-DR beta-chain cDNA sequences - PubMed (original) (raw)

Evolutionary and genetic implications of sequence variation in two nonallelic HLA-DR beta-chain cDNA sequences

J M Curtsinger et al. Proc Natl Acad Sci U S A. 1987 Jan.

Abstract

Most HLA haplotypes carry two expressed DR beta-chain genes; in the DR4 haplotype, the polymorphic locus has been called DR beta 1 and the apparently nonpolymorphic locus has been called DR beta 2. We have isolated nearly full-length DR beta-chain cDNA clones representing each of these two loci from a cell line homozygous for DR4 and Dw4. The clones have been sequenced and the sequences compared with published DR beta cDNA sequences derived from other haplotypes. A comparison of our sequences with other published cDNA sequences did not allow assignment of these other sequences to either the beta 1 or beta 2 locus. Comparison of our DR4 beta 1 sequence with DR beta 1 sequences isolated from other DR4-positive cells suggests that the alleles of DR4 beta 1 may have recently diverged from a common ancestor. The apparent lack of polymorphism of DR beta 2 may in part be a reflection of this recent divergence.

PubMed Disclaimer

References

    1. EMBO J. 1984 Sep;3(9):2029-32 - PubMed
    1. J Exp Med. 1984 Sep 1;160(3):751-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Mar;82(5):1475-9 - PubMed
    1. Plasmid. 1985 Jan;13(1):31-40 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 May;82(10):3405-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources