Induction of casein kinase II during differentiation of 3T3-L1 cells - PubMed (original) (raw)
. 1987 Mar 15;262(8):3839-43.
- PMID: 3469203
Free article
Induction of casein kinase II during differentiation of 3T3-L1 cells
J Sommercorn et al. J Biol Chem. 1987.
Free article
Abstract
The peptide Arg-Arg-Arg-Glu-Glu-Glu-Thr-Glu-Glu-Glu was shown to be a specific substrate for casein kinase II (CK II) in extracts of 3T3-L1 cells. Fractionation of a cell extract on DEAE-cellulose revealed only one peptide kinase and it eluted at the same salt concentration required to elute CK II. Consistent with the properties of CK II, the peptide kinase activity was inhibited by very low concentrations of heparin (Ki less than 6 nM) and it used GTP efficiently as a substrate. A Western blot, developed with antiserum to bovine thymus CK II, demonstrated the presence of CK II protein in 3T3-L1 extracts and that peptide kinase activity was directly related to the amount of CK II protein. The peptide was used to assay CK II activity in extracts of 3T3-L1 cells stimulated to differentiate into adipocytes. Differentiation produced a transient increase in CK II activity that reached a maximum (4-fold) on day 4. The increased activity was accounted for by increased CK II protein. Induction of CK II preceded the increase in total protein and was not the result of cell proliferation. CK II induction was coincident with induction of the insulin receptor, but, whereas insulin binding remained elevated, CK II activity declined after day 4. Agents that stimulate differentiation of 3T3-L1 cells did not cause induction of CK II in 3T3-C2 cells that do not differentiate. The transient nature of the induction of CK II suggests that the kinase may contribute to the process of differentiation rather than being a phenotypic change like that of the insulin receptor.
Similar articles
- Insulin-like growth factor I and insulin rapidly increase casein kinase II activity in BALB/c 3T3 fibroblasts.
Klarlund JK, Czech MP. Klarlund JK, et al. J Biol Chem. 1988 Nov 5;263(31):15872-5. J Biol Chem. 1988. PMID: 3053682 - Rapid stimulation of Ser/Thr protein kinases following treatment of Swiss 3T3 cells with bombesin. Involvement of casein kinase-2 in the signaling pathway of bombesin.
Agostinis P, Van Lint J, Sarno S, De Witte P, Vandenheede JR, Merlevede W. Agostinis P, et al. J Biol Chem. 1992 May 15;267(14):9732-7. J Biol Chem. 1992. PMID: 1577810 - Ribosomal proteins P0, P1, and P2 are phosphorylated by casein kinase II at their conserved carboxyl termini.
Hasler P, Brot N, Weissbach H, Parnassa AP, Elkon KB. Hasler P, et al. J Biol Chem. 1991 Jul 25;266(21):13815-20. J Biol Chem. 1991. PMID: 1856214 - Stimulation of FA and casein kinase II by insulin in 3T3-L1 cells.
Villa-Moruzzi E, Crabb JW. Villa-Moruzzi E, et al. Biochem Biophys Res Commun. 1991 Jun 28;177(3):1019-24. doi: 10.1016/0006-291x(91)90640-s. Biochem Biophys Res Commun. 1991. PMID: 1647765 - The insulin/Ras pathway of adipocytic differentiation of 3T3 L1 cells: dissociation between Raf-1 kinase and the MAPK/RSK cascade.
Porras A, Santos E. Porras A, et al. Int J Obes Relat Metab Disord. 1996 Mar;20 Suppl 3:S43-51. Int J Obes Relat Metab Disord. 1996. PMID: 8680477 Review.
Cited by
- ROCK2-Specific Inhibitor KD025 Suppresses Adipocyte Differentiation by Inhibiting Casein Kinase 2.
Tran NNQ, Chun KH. Tran NNQ, et al. Molecules. 2021 Aug 5;26(16):4747. doi: 10.3390/molecules26164747. Molecules. 2021. PMID: 34443331 Free PMC article. - Protein kinase CK2 in development and differentiation.
Götz C, Montenarh M. Götz C, et al. Biomed Rep. 2017 Feb;6(2):127-133. doi: 10.3892/br.2016.829. Epub 2016 Dec 19. Biomed Rep. 2017. PMID: 28357063 Free PMC article. - Drosophila Protein Kinase CK2: Genetics, Regulatory Complexity and Emerging Roles during Development.
Bandyopadhyay M, Arbet S, Bishop CP, Bidwai AP. Bandyopadhyay M, et al. Pharmaceuticals (Basel). 2016 Dec 29;10(1):4. doi: 10.3390/ph10010004. Pharmaceuticals (Basel). 2016. PMID: 28036067 Free PMC article. Review. - Glutamate Counteracts Dopamine/PKA Signaling via Dephosphorylation of DARPP-32 Ser-97 and Alteration of Its Cytonuclear Distribution.
Nishi A, Matamales M, Musante V, Valjent E, Kuroiwa M, Kitahara Y, Rebholz H, Greengard P, Girault JA, Nairn AC. Nishi A, et al. J Biol Chem. 2017 Jan 27;292(4):1462-1476. doi: 10.1074/jbc.M116.752402. Epub 2016 Dec 20. J Biol Chem. 2017. PMID: 27998980 Free PMC article. - Protein kinase CK2 is implicated in early steps of the differentiation of pre-adipocytes into adipocytes.
Wilhelm N, Kostelnik K, Götz C, Montenarh M. Wilhelm N, et al. Mol Cell Biochem. 2012 Jun;365(1-2):37-45. doi: 10.1007/s11010-012-1241-y. Epub 2012 Jan 24. Mol Cell Biochem. 2012. PMID: 22270543
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous