Cloning of a brain protein identified by autoantibodies from a patient with paraneoplastic cerebellar degeneration - PubMed (original) (raw)

Comparative Study

Cloning of a brain protein identified by autoantibodies from a patient with paraneoplastic cerebellar degeneration

E J Dropcho et al. Proc Natl Acad Sci U S A. 1987 Jul.

Abstract

Autoantibodies directed against neuronal proteins have been identified in some patients with paraneoplastic cerebellar degeneration. To identify the molecular targets for these autoantibodies, we constructed a lambda gt11 cDNA expression library from human cerebellum and screened the library with IgG from a patient with paraneoplastic cerebellar degeneration. A single clone, pCDR2, produced a fusion protein that reacted strongly with the patient's IgG. The isolated pCDR2 clone was used to identify six overlapping cDNA clones. Sequencing of the pCDR clones revealed a distinctive pattern consisting of a unit of 18 nucleotides (6 amino acids) repeated in tandem along the entire cDNA sequence. This sequence is unlike any previously described eukaryotic gene. Southern blot analysis was consistent with single-copy representation of the CDR (cerebellar degeneration-related) gene in the human and mouse genome. RNA blotting studies with normal tissues showed expression of the CDR gene to be largely restricted to brain. Expression of the CDR message was also noted in cell lines derived from cancers of neuroectodermal, kidney, and lung origin.

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References

    1. Brain. 1965 Sep;88(3):465-78 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6450-4 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Arch Neurol. 1976 Sep;33(9):660-1 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed

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