Analysis of the tissue-specific enhancer at the 3' end of the chicken adult beta-globin gene - PubMed (original) (raw)

Analysis of the tissue-specific enhancer at the 3' end of the chicken adult beta-globin gene

B M Emerson et al. Proc Natl Acad Sci U S A. 1987 Jul.

Abstract

In an earlier paper we identified a tissue-specific enhancer in the 3' flanking region of the chicken adult beta-globin gene. In this paper we analyze the properties of this enhancer. Deletion analysis and transient expression assays show that the domain responsible for activation of transcription is at most 136 base pairs long. Specific factors that bind to discrete sequences within the enhancer DNA are found in extracts of embryonic and adult erythrocytes and in brain. These factors are specific for the tissue or the erythrocyte developmental stage and protect at least five discrete regions in or near the enhancer against DNase I digestion in "footprinting" experiments. Four of these regions reside wholly within the 136-base-pair functional enhancer domain, which also comprises a site in chromatin that is hypersensitive to nucleases. The nature of the binding sites and the program of appearance of the factors during development suggest that a subset of these interactions may be responsible for the developmental specificity of the enhancer.

PubMed Disclaimer

Similar articles

• An erythrocyte-specific DNA-binding factor recognizes a regulatory sequence common to all chicken globin genes.
  Evans T, Reitman M, Felsenfeld G. Evans T, et al. Proc Natl Acad Sci U S A. 1988 Aug;85(16):5976-80. doi: 10.1073/pnas.85.16.5976. Proc Natl Acad Sci U S A. 1988. PMID: 3413070 Free PMC article.
• CTCF-dependent enhancer blockers at the upstream region of the chicken alpha-globin gene domain.
  Valadez-Graham V, Razin SV, Recillas-Targa F. Valadez-Graham V, et al. Nucleic Acids Res. 2004 Feb 23;32(4):1354-62. doi: 10.1093/nar/gkh301. Print 2004. Nucleic Acids Res. 2004. PMID: 14981153 Free PMC article.
• Identification of functional elements of the chicken epsilon-globin promoter involved in stage-specific interaction with the beta/epsilon enhancer.
  Mason MM, Grasso JA, Gavrilova O, Reitman M. Mason MM, et al. J Biol Chem. 1996 Oct 11;271(41):25459-67. doi: 10.1074/jbc.271.41.25459. J Biol Chem. 1996. PMID: 8810316
• Chromatin domains and regulation of gene expression: familiar and enigmatic clusters of chicken globin genes.
  Recillas-Targa F, Razin SV. Recillas-Targa F, et al. Crit Rev Eukaryot Gene Expr. 2001;11(1-3):227-42. Crit Rev Eukaryot Gene Expr. 2001. PMID: 11693962 Review.
• cis and trans regulation of tissue-specific transcription.
  Engel JD, Beug H, LaVail JH, Zenke MW, Mayo K, Leonard MW, Foley KP, Yang Z, Kornhauser JM, Ko LJ, et al. Engel JD, et al. J Cell Sci Suppl. 1992;16:21-31. doi: 10.1242/jcs.1992.supplement_16.4. J Cell Sci Suppl. 1992. PMID: 1297649 Review.

Cited by

• Stepwise activation of the mouse acetylcholine receptor delta- and gamma-subunit genes in clonal cell lines.
  Crowder CM, Merlie JP. Crowder CM, et al. Mol Cell Biol. 1988 Dec;8(12):5257-67. doi: 10.1128/mcb.8.12.5257-5267.1988. Mol Cell Biol. 1988. PMID: 3244354 Free PMC article.
• Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene.
  Mignotte V, Wall L, deBoer E, Grosveld F, Romeo PH. Mignotte V, et al. Nucleic Acids Res. 1989 Jan 11;17(1):37-54. doi: 10.1093/nar/17.1.37. Nucleic Acids Res. 1989. PMID: 2911469 Free PMC article.
• Endothelial-cell-specific regulation of von Willebrand factor gene expression.
  Jahroudi N, Lynch DC. Jahroudi N, et al. Mol Cell Biol. 1994 Feb;14(2):999-1008. doi: 10.1128/mcb.14.2.999-1008.1994. Mol Cell Biol. 1994. PMID: 7507210 Free PMC article.
• Lability of leukosis virus enhancer-binding proteins in avian hematopoeitic cells.
  Ruddell A, Linial M, Schubach W, Groudine M. Ruddell A, et al. J Virol. 1988 Aug;62(8):2728-35. doi: 10.1128/JVI.62.8.2728-2735.1988. J Virol. 1988. PMID: 2839698 Free PMC article.
• Multiple regulatory elements of the murine gamma 2-crystallin promoter.
  Lok S, Stevens W, Breitman ML, Tsui LC. Lok S, et al. Nucleic Acids Res. 1989 May 11;17(9):3563-82. doi: 10.1093/nar/17.9.3563. Nucleic Acids Res. 1989. PMID: 2726487 Free PMC article.

References

1. 1. Cell. 1981 Nov;27(1 Pt 2):45-55 - PubMed
2. 1. Protein Eng. 1986 Oct-Nov;1(1):67-74 - PubMed
3. 1. Mol Cell Biol. 1982 Sep;2(9):1044-51 - PubMed
4. 1. Cell. 1983 Jun;33(2):313-4 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1983 Oct;80(20):6177-81 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central