Production of granulocyte-macrophage colony-stimulating factor by Abelson virus-induced tumorigenic mast cell lines - PubMed (original) (raw)
. 1986 Nov;68(5):1074-81.
- PMID: 3490285
Free article
Production of granulocyte-macrophage colony-stimulating factor by Abelson virus-induced tumorigenic mast cell lines
S W Chung et al. Blood. 1986 Nov.
Free article
Abstract
We have recently described a system that supports the development of continuously growing and tumorigenic cell lines after infection of individual multilineage hematopoietic colonies with Abelson murine leukemia virus (A-MuLV). We now provide definitive evidence that these transformed lines express features characteristic of mast cells. Although these lines have been maintained in some cases for more than a year in the absence of exogenous growth factors other than those present in fetal calf serum, colony formation could consistently after 2 months, and variably after 5 months, be shown to be increased several fold when pokeweed mitogen-stimulated spleen cell conditioned medium (CM) was added to the cultures. CM from the A-MuLV-transformed lines was then tested for its ability to stimulate hematopoietic colony formation by cells from both fetal and adult tissues. Four of four randomly selected cell lines produced factors that were active on erythropoietic, granulopoietic, and in some cases pluripotent progenitors. Removal of viral particles from the CM from one of the lines (27d1) by either heat inactivation or high-speed centrifugation did not alter the colony-stimulating activity detected. When CM from 27d1 cells was tested for its ability to stimulate the proliferation of interleukin 3 (IL3) granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent FDC-P1 cells, a positive result was obtained. This stimulatory activity was not reduced in the presence of neutralizing anti-IL 3 immunoglobulin (Ig), suggesting that the activity detected was GM-CSF and not IL 3. This was confirmed by the lack of expression of the IL 3 gene in 27d1 cells as determined by Northern analysis of 27d1 cell RNA. Furthermore, S1 analysis of mRNA from 27d1 cells as well as two other lines indicated that the GM-CSF gene in all three was transcriptionally active. Taken together, these data suggest that A-MuLV transformation of normal mast cells or their precursors under certain conditions commonly activates the production of GM-CSF.
Similar articles
- Activation of multiple hemopoietic growth factor genes in Abelson virus-transformed myeloid cells.
Humphries RK, Abraham S, Krystal G, Lansdorp P, Lemoine F, Eaves CJ. Humphries RK, et al. Exp Hematol. 1988 Oct;16(9):774-81. Exp Hematol. 1988. PMID: 2844575 - Friend murine leukemia virus-immortalized myeloid cells are converted into tumorigenic cell lines by Abelson leukemia virus.
Oliff A, Agranovsky O, McKinney MD, Murty VV, Bauchwitz R. Oliff A, et al. Proc Natl Acad Sci U S A. 1985 May;82(10):3306-10. doi: 10.1073/pnas.82.10.3306. Proc Natl Acad Sci U S A. 1985. PMID: 2987918 Free PMC article.
Cited by
- Leukemia initiated by hemopoietic stem cells expressing the v-abl oncogene.
Chung SW, Wong PM, Durkin H, Wu YS, Petersen J. Chung SW, et al. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1585-9. doi: 10.1073/pnas.88.4.1585. Proc Natl Acad Sci U S A. 1991. PMID: 1996361 Free PMC article. - High-efficiency identification of genes by functional analysis from a retroviral cDNA expression library.
Wong BY, Chen H, Chung SW, Wong PM. Wong BY, et al. J Virol. 1994 Sep;68(9):5523-31. doi: 10.1128/JVI.68.9.5523-5531.1994. J Virol. 1994. PMID: 8057434 Free PMC article. - Mast Cell Responses to Viruses and Pathogen Products.
Marshall JS, Portales-Cervantes L, Leong E. Marshall JS, et al. Int J Mol Sci. 2019 Aug 30;20(17):4241. doi: 10.3390/ijms20174241. Int J Mol Sci. 2019. PMID: 31480219 Free PMC article. Review. - Mast cells as sources of cytokines, chemokines, and growth factors.
Mukai K, Tsai M, Saito H, Galli SJ. Mukai K, et al. Immunol Rev. 2018 Mar;282(1):121-150. doi: 10.1111/imr.12634. Immunol Rev. 2018. PMID: 29431212 Free PMC article. Review. - Chronic myeloproliferative disease induced by site-specific integration of Abelson murine leukemia virus-infected hemopoietic stem cells.
Han XD, Wong PM, Chung SW. Han XD, et al. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10129-33. doi: 10.1073/pnas.88.22.10129. Proc Natl Acad Sci U S A. 1991. PMID: 1682923 Free PMC article.