Plasmid establishment in competent Haemophilus influenzae occurs by illegitimate transformation - PubMed (original) (raw)

Plasmid establishment in competent Haemophilus influenzae occurs by illegitimate transformation

M L Pifer. J Bacteriol. 1986 Nov.

Abstract

Establishment of plasmids in naturally competent Haemophilus influenzae is incompatible with transformation via the normal DNA translocation pathways. Instead, establishing plasmids appear to evade the degradation which ordinarily accompanies translocation, arriving as intact double-stranded molecules in the cytoplasm. Evidence is presented that plasmid establishment is a rare illegitimate transformation event which resembles artificial transformation. This process is compared with plasmid marker rescue transformation, and a method for greatly increasing establishment frequency is described.

PubMed Disclaimer

References

1. 1. Gene. 1980 Nov;11(3-4):311-8 -PubMed
2. 1. J Bacteriol. 1980 Dec;144(3):999-1002 -PubMed
3. 1. J Bacteriol. 1981 May;146(2):564-70 -PubMed
4. 1. Annu Rev Biochem. 1981;50:41-68 -PubMed
5. 1. J Bacteriol. 1981 Dec;148(3):812-6 -PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PMC
  • PubMed Central