Posttranscriptional gene regulation and specific binding of the nonhistone protein HMG-I by the 3' untranslated region of bovine interleukin 2 cDNA - PubMed (original) (raw)

Posttranscriptional gene regulation and specific binding of the nonhistone protein HMG-I by the 3' untranslated region of bovine interleukin 2 cDNA

R Reeves et al. Proc Natl Acad Sci U S A. 1987 Sep.

Abstract

The 3' untranslated tail region (3'-UTR) of the cDNA of bovine interleukin 2 (bIL-2) acts as a lymphoid cell-specific gene regulatory element in vivo when ligated to the 3' end of the "marker" bacterial gene coding for chloramphenicol acetyltransferase (CAT) and the hybrid fusion gene is introduced into bovine lymphoid cells by transfection. Evidence is also presented that the 3'-UTR with its conserved (TATT)n motif probably has multiple functions in lymphoid cells operating both at the chromosomal level, where the sequence may be involved in the specific binding of the nonhistone chromatin high mobility group protein HMG-I, and at the RNA level, where the conserved sequence is involved in selective posttranscriptional mRNA degradation by a lymphocyte-specific nuclease(s). These results suggest a complex in vivo role for the 3'-UTR of bIL-2 cDNA and the conserved (TATT)n sequences found within it. They also offer a plausible explanation for the high degree of conservation of similar A + T-rich sequences in the 3'-UTRs of many of the other immune-response and growth-regulatory genes of mammals.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Cell. 1985 Jun;41(2):497-507 - PubMed
    1. Cell. 1985 Jun;41(2):509-20 - PubMed
    1. J Exp Med. 1985 Jun 1;161(6):1593-8 - PubMed
    1. Nucleic Acids Res. 1985 May 24;13(10):3599-615 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Aug;82(15):4987-91 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources