Rapid decrease in lymphocyte adherence to high endothelial venules in lymph nodes deprived of afferent lymphatic vessels - PubMed (original) (raw)
Rapid decrease in lymphocyte adherence to high endothelial venules in lymph nodes deprived of afferent lymphatic vessels
H R Hendriks et al. Eur J Immunol. 1987 Dec.
Abstract
Occlusion of the afferent lymph flow to the lymph node (LN) results in both flattening of the endothelium of high endothelial venules (HEV) and a severe decrease in numbers of lymphocytes in transit across the walls of the flattened HEV. In the present study we have used the in vitro lymphocyte-binding assay to investigate the ability of HEV in rat LN to bind lymphocytes at various time points after occlusion of the afferent lymph flow. In addition the specificity of T and B lymphocyte adherence to HEV of such operated LN was studied. In normal LN, lymphocytes adhered to virtually all HEV using the in vitro binding assay. However, 1 and 2 weeks after operation lymphocytes bound to only 50-60% of the HEV and by 3-6 weeks 20-30%. The total numbers of lymphocytes bound to these HEV had also diminished to 10% of the control value 3-6 weeks after operation. Morphometric analysis showed that this was not only due to a reduction in the area of HEV endothelium available for lymphocyte adherence by flattening of the high endothelial cells, but also to a strong decrease in the numbers of bound lymphocytes per unit area high endothelium. In spite of the reduction in numbers of adhering lymphocytes the T/B cell ratio did not change. The results show that the reduction in lymphocyte binding of HEV in operated LN is a rapid event, probably due to loss of high endothelial cell determinants involved in binding of lymphocytes. The decrease in lymphocyte binding clearly precedes flattening of HEV endothelium suggesting that the height of high endothelial cells is of secondary importance to lymphocyte adherence.
Similar articles
- Interaction of B and T lymphocyte subsets with high endothelial venules in the rat: binding in vitro does not reflect homing in vivo.
Walter S, Micheel B, Pabst R, Westermann J. Walter S, et al. Eur J Immunol. 1995 May;25(5):1199-205. doi: 10.1002/eji.1830250510. Eur J Immunol. 1995. PMID: 7774623 - Specific lymphocyte-endothelial cell interactions regulate migration into lymph nodes, Peyer's patches, and skin.
Chin YH, Falanga V, Streilein JW, Sackstein R. Chin YH, et al. Reg Immunol. 1988 Jul-Aug;1(1):78-83. Reg Immunol. 1988. PMID: 3079311 Review. - Neogenesis and development of the high endothelial venules that mediate lymphocyte trafficking.
Hayasaka H, Taniguchi K, Fukai S, Miyasaka M. Hayasaka H, et al. Cancer Sci. 2010 Nov;101(11):2302-8. doi: 10.1111/j.1349-7006.2010.01687.x. Cancer Sci. 2010. PMID: 20726857 Free PMC article. Review.
Cited by
- Loss of direct adrenergic innervation after peripheral nerve injury causes lymph node expansion through IFN-γ.
Chen CS, Weber J, Holtkamp SJ, Ince LM, de Juan A, Wang C, Lutes L, Barnoud C, Kizil B, Hergenhan SM, Salvermoser J, Lasch M, Deindl E, Schraml B, Baumjohann D, Scheiermann C. Chen CS, et al. J Exp Med. 2021 Aug 2;218(8):e20202377. doi: 10.1084/jem.20202377. Epub 2021 Jun 4. J Exp Med. 2021. PMID: 34086056 Free PMC article. - High endothelial venules (HEVs) in immunity, inflammation and cancer.
Blanchard L, Girard JP. Blanchard L, et al. Angiogenesis. 2021 Nov;24(4):719-753. doi: 10.1007/s10456-021-09792-8. Epub 2021 May 6. Angiogenesis. 2021. PMID: 33956259 Free PMC article. Review. - Lymph node stromal cells: cartographers of the immune system.
Krishnamurty AT, Turley SJ. Krishnamurty AT, et al. Nat Immunol. 2020 Apr;21(4):369-380. doi: 10.1038/s41590-020-0635-3. Epub 2020 Mar 23. Nat Immunol. 2020. PMID: 32205888 Review. - Mechanosensing by Peyer's patch stroma regulates lymphocyte migration and mucosal antibody responses.
Chang JE, Buechler MB, Gressier E, Turley SJ, Carroll MC. Chang JE, et al. Nat Immunol. 2019 Nov;20(11):1506-1516. doi: 10.1038/s41590-019-0505-z. Epub 2019 Oct 14. Nat Immunol. 2019. PMID: 31611698 Free PMC article. - Bimodal Expansion of the Lymphatic Vessels Is Regulated by the Sequential Expression of IL-7 and Lymphotoxin α1β2 in Newly Formed Tertiary Lymphoid Structures.
Nayar S, Campos J, Chung MM, Navarro-Núñez L, Chachlani M, Steinthal N, Gardner DH, Rankin P, Cloake T, Caamaño JH, McGettrick HM, Watson SP, Luther S, Buckley CD, Barone F. Nayar S, et al. J Immunol. 2016 Sep 1;197(5):1957-67. doi: 10.4049/jimmunol.1500686. Epub 2016 Jul 29. J Immunol. 2016. PMID: 27474071 Free PMC article.
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources