Isolation and morphological characterization of a mycelial mutant of Candida albicans - PubMed (original) (raw)

Isolation and morphological characterization of a mycelial mutant of Candida albicans

M J Hubbard et al. J Bacteriol. 1986 Jan.

Abstract

In this paper we describe the isolation of a novel strain of Candida albicans which is a mycelium at ambient temperatures. Mutagenesis of C. albicans ATCC 10261 with N-methyl-N-nitro-N-nitrosoguanidine followed by plating on solid media at 28 degrees C yielded colony morphology variants which were characterized by a raised, rough-surfaced colony of irregular outline in marked contrast to the flat, shiny circular colonies of the parental 10261 strain. One mutant colony, hOG301, was studied in detail. Strain hOG301 was stable and exhibited mycelial morphology over a wide temperature range (5 to 40 degrees C) in several media. The hyphae comprising hOG301 mycelium were examined by light microscopy, scanning electron microscopy, and transmission electron microscopy and showed morphological features described in the literature as being typical of both true hyphae and pseudohyphae. In contrast to 10261, hOG301 was not pathogenic after intraperitoneal injection in mice. This is the first report of a mycelial C. albicans that is stable at ambient temperatures.

PubMed Disclaimer

References

    1. J Cell Biol. 1971 Jan;48(1):192-7 - PubMed
    1. Sabouraudia. 1973 Nov;11(3):263-6 - PubMed
    1. J Bacteriol. 1981 Jun;146(3):833-40 - PubMed
    1. Med Lab Sci. 1981 Jul;38(3):237-44 - PubMed
    1. J Bacteriol. 1982 Dec;152(3):969-75 - PubMed

Publication types

MeSH terms

LinkOut - more resources