Immunocytochemical evidence for phorbol ester-induced protein kinase C translocation in HL60 cells - PubMed (original) (raw)
Immunocytochemical evidence for phorbol ester-induced protein kinase C translocation in HL60 cells
M Shoji et al. Biochem Biophys Res Commun. 1986.
Abstract
The ability of tumor promoting 12-O-tetradecanoylphorbol-13-acetate (TPA) to redistribute protein kinase C in human promyelocytic leukemic HL60 cells was investigated. It was found that TPA caused a rapid translocation (within 10 min) of protein kinase C from the cytosolic (soluble) fraction to the particulate (membrane) fraction, as determined indirectly by assaying for the enzyme activity or by immunoblotting of the enzyme protein in the isolated subcellular fractions. Immunocytochemical localization of the enzyme demonstrated directly that the TPA caused an enzyme translocation t the plasma membrane. These findings suggest that translocation to the plasma membrane of the enzyme may represent initial events related to the TPA effect on terminal differentiation of HL60 cells to monocytes/macrophages.
Similar articles
- Phytohemagglutinin treatment of T lymphocytes stimulates rapid increases in activity of both particulate and cytosolic protein kinase C.
Mire AR, Wickremasinghe RG, Hoffbrand AV. Mire AR, et al. Biochem Biophys Res Commun. 1986 May 29;137(1):128-34. doi: 10.1016/0006-291x(86)91185-x. Biochem Biophys Res Commun. 1986. PMID: 3487317 - Protein phosphorylation events and changes in inositol metabolism during HL60 cell differentiation.
Lord JM, Bunce CM, French PJ, Jones NA, Baxter MA, Grafton G, Michell RH, Brown G. Lord JM, et al. Biochem Soc Trans. 1991 Apr;19(2):315-20. doi: 10.1042/bst0190315. Biochem Soc Trans. 1991. PMID: 1889612 Review. No abstract available.
Cited by
- Immunocytochemical evidence for translocation of protein kinase C in human megakaryoblastic leukemic cells: synergistic effects of Ca2+ and activators of protein kinase C on the plasma membrane association.
Ito T, Tanaka T, Yoshida T, Onoda K, Ohta H, Hagiwara M, Itoh Y, Ogura M, Saito H, Hidaka H. Ito T, et al. J Cell Biol. 1988 Sep;107(3):929-37. doi: 10.1083/jcb.107.3.929. J Cell Biol. 1988. PMID: 3047154 Free PMC article. - Characterization of calreticulin as a protein interacting with protein kinase C.
Rendón-Huerta E, Mendoza-Hernández G, Robles-Flores M. Rendón-Huerta E, et al. Biochem J. 1999 Dec 1;344 Pt 2(Pt 2):469-75. Biochem J. 1999. PMID: 10567230 Free PMC article. - Immunogold electron microscopic demonstration of distinct submembranous localization of the activated gammaPKC depending on the stimulation.
Oyasu M, Fujimiya M, Kashiwagi K, Ohmori S, Imaeda H, Saito N. Oyasu M, et al. J Histochem Cytochem. 2008 Mar;56(3):253-65. doi: 10.1369/jhc.7A7291.2007. Epub 2007 Nov 26. J Histochem Cytochem. 2008. PMID: 18040079 Free PMC article. - Effects of hexadecylphosphocholine on protein kinase C and TPA-induced differentiation of HL60 cells.
Shoji M, Raynor RL, Fleer EA, Eibl H, Vogler WR, Kuo JF. Shoji M, et al. Lipids. 1991 Feb;26(2):145-9. doi: 10.1007/BF02544009. Lipids. 1991. PMID: 2051897