Identification of a telomere-binding activity from yeast - PubMed (original) (raw)

Identification of a telomere-binding activity from yeast

J Berman et al. Proc Natl Acad Sci U S A. 1986 Jun.

Abstract

In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to poly(C1-3A) sequences, despite their different primary sequences, yet does not bind specifically to telomeric repeats, such as poly(C4A2), poly(C4A4), and poly (C1-8T) from other lower eukaryotes.

PubMed Disclaimer

Similar articles

• A yeast telomere binding activity binds to two related telomere sequence motifs and is indistinguishable from RAP1.
  Longtine MS, Wilson NM, Petracek ME, Berman J. Longtine MS, et al. Curr Genet. 1989 Oct;16(4):225-39. doi: 10.1007/BF00422108. Curr Genet. 1989. PMID: 2697465
• Telomere-telomere recombination provides an express pathway for telomere acquisition.
  Wang SS, Zakian VA. Wang SS, et al. Nature. 1990 May 31;345(6274):456-8. doi: 10.1038/345456a0. Nature. 1990. PMID: 2111466
• Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation.
  Runge KW, Zakian VA. Runge KW, et al. Mol Cell Biol. 1989 Apr;9(4):1488-97. doi: 10.1128/mcb.9.4.1488-1497.1989. Mol Cell Biol. 1989. PMID: 2657397 Free PMC article.
• The structure and function of yeast centromeres.
  Clarke L, Carbon J. Clarke L, et al. Annu Rev Genet. 1985;19:29-55. doi: 10.1146/annurev.ge.19.120185.000333. Annu Rev Genet. 1985. PMID: 3909945 Review. No abstract available.
• Yeast telomeres: the end of the chromosome story?
  Walmsley RM. Walmsley RM. Yeast. 1987 Sep;3(3):139-48. doi: 10.1002/yea.320030302. Yeast. 1987. PMID: 3332970 Review. No abstract available.

Cited by

• Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states.
  Barry RM, Sacco O, Mameri A, Stojaspal M, Kartsonis W, Shah P, De Ioannes P, Hofr C, Côté J, Sfeir A. Barry RM, et al. Genes Dev. 2022 Mar 1;36(5-6):313-330. doi: 10.1101/gad.349039.121. Epub 2022 Feb 24. Genes Dev. 2022. PMID: 35210222 Free PMC article.
• Either Rap1 or Cdc13 can protect telomeric single-stranded 3' overhangs from degradation in vitro.
  Runnberg R, Narayanan S, Itriago H, Cohn M. Runnberg R, et al. Sci Rep. 2019 Dec 16;9(1):19181. doi: 10.1038/s41598-019-55482-3. Sci Rep. 2019. PMID: 31844093 Free PMC article.
• Double-stranded telomeric DNA binding proteins: Diversity matters.
  Červenák F, Juríková K, Sepšiová R, Neboháčová M, Nosek J, Tomáška L. Červenák F, et al. Cell Cycle. 2017;16(17):1568-1577. doi: 10.1080/15384101.2017.1356511. Epub 2017 Jul 27. Cell Cycle. 2017. PMID: 28749196 Free PMC article. Review.
• Overcoming natural replication barriers: differential helicase requirements.
  Anand RP, Shah KA, Niu H, Sung P, Mirkin SM, Freudenreich CH. Anand RP, et al. Nucleic Acids Res. 2012 Feb;40(3):1091-105. doi: 10.1093/nar/gkr836. Epub 2011 Oct 7. Nucleic Acids Res. 2012. PMID: 21984413 Free PMC article.
• Subtelomeric repetitive elements determine TERRA regulation by Rap1/Rif and Rap1/Sir complexes in yeast.
  Iglesias N, Redon S, Pfeiffer V, Dees M, Lingner J, Luke B. Iglesias N, et al. EMBO Rep. 2011 Jun;12(6):587-93. doi: 10.1038/embor.2011.73. Epub 2011 Apr 28. EMBO Rep. 2011. PMID: 21525956 Free PMC article.

References

1. 1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
2. 1. J Mol Biol. 1978 Mar 25;120(1):33-53 - PubMed
3. 1. Nature. 1984 Jul 12-18;310(5973):154-7 - PubMed
4. 1. Genetics. 1941 Mar;26(2):234-82 - PubMed
5. 1. Nature. 1983 Mar 3;302(5903):84-6 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central