The specific direct interaction of helper T cells and antigen-presenting B cells - PubMed (original) (raw)

The specific direct interaction of helper T cells and antigen-presenting B cells

A Kupfer et al. Proc Natl Acad Sci U S A. 1986 Aug.

Abstract

Cell couples have been formed by mixing an antigen- and Ia-specific cloned helper T-cell line with a B-cell hybridoma presenting the antigen. By immunofluorescence observations, we have shown that the microtubule-organizing center (MTOC) inside the helper T cell, but not in the bound antigen-presenting cell, becomes oriented to face the area of specific cell-cell contact. This MTOC orientation is antigen- and Ia-specific, and thus provides direct evidence for the specific interaction of a helper T cell with a B cell. It is presumed that the function served by this MTOC orientation, which is accompanied by the coordinate reorientation of the Golgi apparatus, is to target Golgi apparatus-derived secretory vesicles, containing putative lymphokines and/or growth factors, from the helper T cell directly to the antigen-presenting cell.

PubMed Disclaimer

Similar articles

• T cell-dependent B cell activation.
  Parker DC. Parker DC. Annu Rev Immunol. 1993;11:331-60. doi: 10.1146/annurev.iy.11.040193.001555. Annu Rev Immunol. 1993. PMID: 8476565 Review.
• Regulation of microtubule-organizing center orientation and actomyosin cytoskeleton rearrangement during immune interactions.
  Sancho D, Vicente-Manzanares M, Mittelbrunn M, Montoya MC, Gordón-Alonso M, Serrador JM, Sánchez-Madrid F. Sancho D, et al. Immunol Rev. 2002 Nov;189:84-97. doi: 10.1034/j.1600-065x.2002.18908.x. Immunol Rev. 2002. PMID: 12445267 Review.

Cited by

• Visualization of Cell-Cell Interaction Contacts: Synapses and Kinapses.
  Dustin ML. Dustin ML. Self Nonself. 2011 Apr;2(2):85-97. doi: 10.4161/self.2.2.17931. Epub 2011 Apr 1. Self Nonself. 2011. PMID: 22299060 Free PMC article.
• The Cbk1p pathway is important for polarized cell growth and cell separation in Saccharomyces cerevisiae.
  Bidlingmaier S, Weiss EL, Seidel C, Drubin DG, Snyder M. Bidlingmaier S, et al. Mol Cell Biol. 2001 Apr;21(7):2449-62. doi: 10.1128/MCB.21.7.2449-2462.2001. Mol Cell Biol. 2001. PMID: 11259593 Free PMC article.
• Recruitment of dynein to the Jurkat immunological synapse.
  Combs J, Kim SJ, Tan S, Ligon LA, Holzbaur EL, Kuhn J, Poenie M. Combs J, et al. Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14883-8. doi: 10.1073/pnas.0600914103. Epub 2006 Sep 21. Proc Natl Acad Sci U S A. 2006. PMID: 16990435 Free PMC article.
• Ultrastructural Study of Cryptococcus neoformans Surface During Budding Events.
  Araújo GRS, Alcantara CL, Rodrigues N, de Souza W, Pontes B, Frases S. Araújo GRS, et al. Front Microbiol. 2021 Mar 1;12:609244. doi: 10.3389/fmicb.2021.609244. eCollection 2021. Front Microbiol. 2021. PMID: 33732220 Free PMC article.
• CD22-mediated stimulation of T cells regulates T-cell receptor/CD3-induced signaling.
  Aruffo A, Kanner SB, Sgroi D, Ledbetter JA, Stamenkovic I. Aruffo A, et al. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10242-6. doi: 10.1073/pnas.89.21.10242. Proc Natl Acad Sci U S A. 1992. PMID: 1438211 Free PMC article.

References

1. 1. J Exp Med. 1981 May 1;153(5):1198-214 - PubMed
2. 1. Immunol Rev. 1981;54:187-223 - PubMed
3. 1. J Exp Med. 1982 Mar 1;155(3):914-23 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2603-7 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3604-7 - PubMed

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central