Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures - PubMed (original) (raw)

Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures

T Nakamura et al. Proc Natl Acad Sci U S A. 1986 Sep.

Abstract

A growth factor (HGF) stimulating DNA synthesis of adult rat hepatocytes in primary culture was found in rat platelets. HGF was purified from rat platelets to homogeneity by a three-step procedure: stimulation of its release from platelets by thrombin, cation-exchanger fast protein liquid chromatography on a Mono S column, and heparin-Sepharose chromatography. HGF was clearly distinguishable from the platelet-derived growth factor (PDGF) by fast protein liquid chromatography. HGF was a heat- and acid-labile cationic protein that was inactivated by reduction with dithiothreitol. Its molecular mass was estimated to be 27 kDa by NaDodSO4/PAGE and its amino acid composition was very different from that of PDGF. The purified HGF stimulated DNA synthesis in adult rat hepatocytes at 2 ng/ml and was maximally effective at 20 ng/ml; its effect was additive or synergistic with those of insulin and EGF, depending on their combinations. HGF did not stimulate DNA synthesis of Swiss 3T3 cells, while PDGF did not stimulate that of hepatocytes. Thus, HGF showed clearly different cell specificity from PDGF in its growth-promoting activities. These findings indicate that HGF is a growth factor in platelets for mature hepatocytes.

PubMed Disclaimer

Similar articles

• Purification and subunit structure of hepatocyte growth factor from rat platelets.
  Nakamura T, Nawa K, Ichihara A, Kaise N, Nishino T. Nakamura T, et al. FEBS Lett. 1987 Nov 30;224(2):311-6. doi: 10.1016/0014-5793(87)80475-1. FEBS Lett. 1987. PMID: 3319692
• Two types of growth inhibitor in rat platelets for primary cultured rat hepatocytes.
  Nakamura T, Teramoto H, Tomita Y, Ichihara A. Nakamura T, et al. Biochem Biophys Res Commun. 1986 Jan 29;134(2):755-63. doi: 10.1016/s0006-291x(86)80485-5. Biochem Biophys Res Commun. 1986. PMID: 3511909
• Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats.
  Nakamura T, Nawa K, Ichihara A. Nakamura T, et al. Biochem Biophys Res Commun. 1984 Aug 16;122(3):1450-9. doi: 10.1016/0006-291x(84)91253-1. Biochem Biophys Res Commun. 1984. PMID: 6477569
• Hepatocyte growth factor: molecular structure and implications for a central role in liver regeneration.
  Matsumoto K, Nakamura T. Matsumoto K, et al. J Gastroenterol Hepatol. 1991 Sep-Oct;6(5):509-19. doi: 10.1111/j.1440-1746.1991.tb00897.x. J Gastroenterol Hepatol. 1991. PMID: 1834243 Review.
• Hepatocyte growth factor: molecular structure, roles in liver regeneration, and other biological functions.
  Matsumoto K, Nakamura T. Matsumoto K, et al. Crit Rev Oncog. 1992;3(1-2):27-54. Crit Rev Oncog. 1992. PMID: 1312869 Review.

Cited by

• Serum hepatocyte growth factor and cancer mortality in an apparently healthy Japanese population.
  Otsuka M, Adachi H, Jacobs DR Jr, Hirai Y, Enomoto M, Fukami A, Kumagae S, Nanjo Y, Yoshikawa K, Esaki E, Kumagai E, Yokoi K, Ogata K, Tsukagawa E, Kasahara A, Ohbu K, Imaizumi T. Otsuka M, et al. J Epidemiol. 2012;22(5):395-401. doi: 10.2188/jea.je20110121. Epub 2012 May 19. J Epidemiol. 2012. PMID: 22672958 Free PMC article.
• Purification and characterization of biologically active scatter factor from ras-transformed NIH 3T3 conditioned medium.
  Coffer A, Fellows J, Young S, Pappin D, Rahman D. Coffer A, et al. Biochem J. 1991 Aug 15;278 ( Pt 1)(Pt 1):35-41. doi: 10.1042/bj2780035. Biochem J. 1991. PMID: 1831975 Free PMC article.
• The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine.
  Nakamura T, Mizuno S. Nakamura T, et al. Proc Jpn Acad Ser B Phys Biol Sci. 2010;86(6):588-610. doi: 10.2183/pjab.86.588. Proc Jpn Acad Ser B Phys Biol Sci. 2010. PMID: 20551596 Free PMC article. Review.
• Functional oesophageal epithelial defense against acid.
  Fujiwara Y, Higuchi K, Tominaga K, Watanabe T, Oshitani N, Arakawa T. Fujiwara Y, et al. Inflammopharmacology. 2005;13(1-3):1-13. doi: 10.1163/156856005774423953. Inflammopharmacology. 2005. PMID: 16259724 Review.
• Production of hepatocyte growth factor during acute myocardial infarction.
  Zhu Y, Hojo Y, Ikeda U, Shimada K. Zhu Y, et al. Heart. 2000 Apr;83(4):450-5. doi: 10.1136/heart.83.4.450. Heart. 2000. PMID: 10722550 Free PMC article.

References

1. 1. Nature. 1970 Jul 11;227(5254):189-90 - PubMed
2. 1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
3. 1. J Biochem. 1978 Oct;84(4):937-46 - PubMed
4. 1. Blood. 1979 Jun;53(6):1043-52 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1980 Oct;77(10):5914-8 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database