Isolation of the yeast calmodulin gene: calmodulin is an essential protein - PubMed (original) (raw)
Isolation of the yeast calmodulin gene: calmodulin is an essential protein
T N Davis et al. Cell. 1986.
Abstract
Calmodulin was purified from Saccharomyces cerevisiae based on its characteristic properties. Like other calmodulins, the yeast protein is small, heat-stable, acidic, retained by hydrophobic matrices in a Ca2+-dependent manner, exhibits a pronounced Ca2+-induced shift in electrophoretic mobility, and binds 45Ca2+. Using synthetic oligonucleotide probes designed from the sequences of two tryptic peptides derived from the purified protein, the gene encoding yeast calmodulin was isolated. The gene (designated CMD1) is a unique, single-copy locus, contains no introns, and resides on chromosome II. The amino acid sequence of yeast calmodulin shares 60% identity with other calmodulins. Disruption or deletion of the yeast calmodulin gene results in a recessive-lethal mutation; thus, calmodulin is essential for the growth of yeast cells.
Similar articles
- Gain-of-function mutations in a human calmodulin-like protein identify residues critical for calmodulin action in yeast.
Harris E, Yaswen P, Thorner J. Harris E, et al. Mol Gen Genet. 1995 Apr 20;247(2):137-47. doi: 10.1007/BF00705643. Mol Gen Genet. 1995. PMID: 7753022 - Isolation of the yeast calmodulin gene using synthetic oligonucleotide probes.
Davis TN, Thorner J. Davis TN, et al. Methods Enzymol. 1987;139:248-62. doi: 10.1016/0076-6879(87)39090-1. Methods Enzymol. 1987. PMID: 3295478 No abstract available. - Identification and characterization of the KlCMD1 gene encoding Kluyveromyces lactis calmodulin.
Rayner TF, Stark MJ. Rayner TF, et al. Yeast. 1998 Jun 30;14(9):869-75. doi: 10.1002/(SICI)1097-0061(19980630)14:9<869::AID-YEA278>3.0.CO;2-U. Yeast. 1998. PMID: 9818725 - Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiae.
Cyert MS. Cyert MS. Annu Rev Genet. 2001;35:647-72. doi: 10.1146/annurev.genet.35.102401.091302. Annu Rev Genet. 2001. PMID: 11700296 Review. - Mutational analysis of calmodulin in Saccharomyces cerevisiae.
Davis TN. Davis TN. Cell Calcium. 1992 Jun-Jul;13(6-7):435-44. doi: 10.1016/0143-4160(92)90056-x. Cell Calcium. 1992. PMID: 1505005 Review.
Cited by
- Cytosolic calcium homeostasis in fungi: roles of plasma membrane transport and intracellular sequestration of calcium.
Miller AJ, Vogg G, Sanders D. Miller AJ, et al. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9348-52. doi: 10.1073/pnas.87.23.9348. Proc Natl Acad Sci U S A. 1990. PMID: 2147513 Free PMC article. - A cytoskeleton-related gene, uso1, is required for intracellular protein transport in Saccharomyces cerevisiae.
Nakajima H, Hirata A, Ogawa Y, Yonehara T, Yoda K, Yamasaki M. Nakajima H, et al. J Cell Biol. 1991 Apr;113(2):245-60. doi: 10.1083/jcb.113.2.245. J Cell Biol. 1991. PMID: 2010462 Free PMC article. - An essential role of the yeast pheromone-induced Ca2+ signal is to activate calcineurin.
Withee JL, Mulholland J, Jeng R, Cyert MS. Withee JL, et al. Mol Biol Cell. 1997 Feb;8(2):263-77. doi: 10.1091/mbc.8.2.263. Mol Biol Cell. 1997. PMID: 9190206 Free PMC article. - Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae.
Flick JS, Thorner J. Flick JS, et al. Mol Cell Biol. 1993 Sep;13(9):5861-76. doi: 10.1128/mcb.13.9.5861-5876.1993. Mol Cell Biol. 1993. PMID: 8395015 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous