Collagen production by rat liver fat-storing cells in primary culture - PubMed (original) (raw)
- PMID: 3536634
- DOI: 10.1159/000163355
Collagen production by rat liver fat-storing cells in primary culture
T Kawase et al. Exp Cell Biol. 1986.
Abstract
Morphological changes, proliferation and collagen synthesis of fat-storing cells (FSC) in primary culture were examined. FSC, isolated from rats treated with vitamin A, showed numerous large lipid droplets in the cytoplasm and positive desmin staining. After 4-7 days culture, these cells were transformed into fibroblast-like cells with a gradual depletion of lipid droplets and with abundant well-developed rough endoplasmic reticulum. The proliferation analysis revealed that DNA synthesis preceded the increase of cell number. Enhancement of the collagen synthesis by FSC were associated with the morphological change of the cells. Quantitative analysis revealed that these cells produced mainly type I collagen (84%) and a small amount of type III collagen (16%).
Similar articles
- Modulation of collagen synthesis by fat-storing cells, isolated from CCl4- or vitamin A-treated rats.
Shiratori Y, Ichida T, Geerts A, Wisse E. Shiratori Y, et al. Dig Dis Sci. 1987 Nov;32(11):1281-9. doi: 10.1007/BF01296379. Dig Dis Sci. 1987. PMID: 3665683 - Culture of proliferating and differentiating fat-storing cells in 3T3-conditioned medium.
Mendoza-Figueroa T, Argüello C, Kuri-Harcuch W. Mendoza-Figueroa T, et al. Biol Cell. 1988;64(1):29-38. doi: 10.1016/0248-4900(88)90090-1. Biol Cell. 1988. PMID: 3224217 - Purified rat liver fat-storing cells in culture divide and contain collagen.
de Leeuw AM, McCarthy SP, Geerts A, Knook DL. de Leeuw AM, et al. Hepatology. 1984 May-Jun;4(3):392-403. doi: 10.1002/hep.1840040307. Hepatology. 1984. PMID: 6373550 - Ultrastructure and function of hepatic fat-storing and pit cells.
Geerts A, Bouwens L, Wisse E. Geerts A, et al. J Electron Microsc Tech. 1990 Mar;14(3):247-56. doi: 10.1002/jemt.1060140306. J Electron Microsc Tech. 1990. PMID: 2187064 Review. - [Structure and function of the fat-storing cell (FSC) in the liver--a review (author's transl)].
Ito T. Ito T. Kaibogaku Zasshi. 1978 Dec;53(6):393-422. Kaibogaku Zasshi. 1978. PMID: 369274 Review. Japanese. No abstract available.
Cited by
- Effects of platelet-derived growth factor and other polypeptide mitogens on DNA synthesis and growth of cultured rat liver fat-storing cells.
Pinzani M, Gesualdo L, Sabbah GM, Abboud HE. Pinzani M, et al. J Clin Invest. 1989 Dec;84(6):1786-93. doi: 10.1172/JCI114363. J Clin Invest. 1989. PMID: 2592560 Free PMC article. - Soluble Arg-Gly-Asp peptides reduce collagen accumulation in cultured rat hepatic stellate cells.
Iwamoto H, Sakai H, Kotoh K, Nakamuta M, Nawata H. Iwamoto H, et al. Dig Dis Sci. 1999 May;44(5):1038-45. doi: 10.1023/a:1026633302985. Dig Dis Sci. 1999. PMID: 10235616 - Collagen measured in primary cultures of normal rat hepatocytes derives from lipocytes within the monolayer.
Maher JJ, Bissell DM, Friedman SL, Roll FJ. Maher JJ, et al. J Clin Invest. 1988 Aug;82(2):450-9. doi: 10.1172/JCI113618. J Clin Invest. 1988. PMID: 3042806 Free PMC article. - Posttranscriptional regulation of collagen alpha1(I) mRNA in hepatic stellate cells.
Stefanovic B, Hellerbrand C, Holcik M, Briendl M, Aliebhaber S, Brenner DA. Stefanovic B, et al. Mol Cell Biol. 1997 Sep;17(9):5201-9. doi: 10.1128/MCB.17.9.5201. Mol Cell Biol. 1997. PMID: 9271398 Free PMC article. - Effects of vitamin A on collagen metabolism by cultured rat liver cells.
Takase S, Enyama K, Takada A, Tsutsumi M. Takase S, et al. Gastroenterol Jpn. 1992 Jun;27(3):354-63. doi: 10.1007/BF02777754. Gastroenterol Jpn. 1992. PMID: 1378030