Functional dissection of Escherichia coli promoters: information in the transcribed region is involved in late steps of the overall process - PubMed (original) (raw)

Functional dissection of Escherichia coli promoters: information in the transcribed region is involved in late steps of the overall process

W Kammerer et al. EMBO J. 1986 Nov.

Abstract

After binding to a promoter Escherichia coli RNA polymerase is in contact with a region of about 70 bp. Around 20 bp of this sequence are transcribed. Information encoded within this transcribed region is involved in late steps of the functional program of a promoter. By changing such 'down-stream' sequences promoter strength in vivo can be varied more than 10-fold. By contrast, information for early steps of the promoter program such as recognition by the enzyme and formation of a stable complex resides in a central core region of about 35 bp. Our data show that the strength of a promoter can be limited at different levels of the overall process. Consequently promoters of identical strength can exhibit different structures due to an alternate optimization of their program.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Nature. 1975 Mar 6;254(5495):34-8 - PubMed
    1. EMBO J. 1986 Nov;5(11):2987-94 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Nucleic Acids Res. 1978 Oct;5(10):3759-73 - PubMed
    1. Nucleic Acids Res. 1979 Jan;6(1):111-37 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources