An accurate, cost-effective and simple colorimetric method for the quantification of total triterpenoid and steroidal saponins from plant materials - PubMed (original) (raw)
An accurate, cost-effective and simple colorimetric method for the quantification of total triterpenoid and steroidal saponins from plant materials
Maxime Le Bot et al. Food Chem. 2022.
Abstract
Saponins are heterosides widely distributed in the plant kingdom. Their properties are used in many industrial sectors, such as food, cosmetics, agriculture, and pharmaceuticals, and their use is increasing due to the market trend to use natural ingredients. Although many techniques exist to quantify saponins (e.g., gravimetric, foaming, spectrophotometric or chromatographic), none of these allow simultaneous accurate, rapid and inexpensive analysis of both triterpenoid and steroidal saponins. A new colorimetric method constituted of p-anisaldehyde and sulfuric acid was developed and avoids all of the above disadvantages. Parameters used in this method allow a similar molar absorptivity for steroidal and triterpenoid saponins with high specificity in complex matrices reducing the sample preparation step and allowing quantification of saponins blends.
Keywords: Chromogenic reaction; Plant extracts; Spectrophotometric quantification; Steroidal saponin; Total saponins; Triterpenoid saponin.
Copyright © 2022 Elsevier Ltd. All rights reserved.
Similar articles
- [The glycosyltransferases involved in triterpenoid saponin biosynthesis: a review].
Zhou C, Gong T, Chen J, Chen T, Yang J, Zhu P. Zhou C, et al. Sheng Wu Gong Cheng Xue Bao. 2022 Mar 25;38(3):1004-1024. doi: 10.13345/j.cjb.210587. Sheng Wu Gong Cheng Xue Bao. 2022. PMID: 35355470 Review. Chinese. - Rapid, cost-effective and accurate quantification of Yucca schidigera Roezl. steroidal saponins using HPLC-ELSD method.
Tenon M, Feuillère N, Roller M, Birtić S. Tenon M, et al. Food Chem. 2017 Apr 15;221:1245-1252. doi: 10.1016/j.foodchem.2016.11.033. Epub 2016 Nov 9. Food Chem. 2017. PMID: 27979085 - Metabolic and functional diversity of saponins, biosynthetic intermediates and semi-synthetic derivatives.
Moses T, Papadopoulou KK, Osbourn A. Moses T, et al. Crit Rev Biochem Mol Biol. 2014 Nov-Dec;49(6):439-62. doi: 10.3109/10409238.2014.953628. Epub 2014 Oct 6. Crit Rev Biochem Mol Biol. 2014. PMID: 25286183 Free PMC article. Review. - P450s and UGTs: Key Players in the Structural Diversity of Triterpenoid Saponins.
Seki H, Tamura K, Muranaka T. Seki H, et al. Plant Cell Physiol. 2015 Aug;56(8):1463-71. doi: 10.1093/pcp/pcv062. Epub 2015 May 6. Plant Cell Physiol. 2015. PMID: 25951908 Free PMC article. Review.
Cited by
- Improvement of triterpenoid production in mycelia of Antrodia camphorata through mutagenesis breeding and amelioration of CCl4-induced liver injury in mice.
Wang HJ, Cui C, Gong XM, Wang S, Li CX, Guo H, Wang YL, Huang YD, Jiang JL, Luo XM, Miao JH, Liu TQ, Zhao S, Feng JX. Wang HJ, et al. Heliyon. 2023 Aug 30;9(9):e19621. doi: 10.1016/j.heliyon.2023.e19621. eCollection 2023 Sep. Heliyon. 2023. PMID: 37809917 Free PMC article. - Cytotoxicity and Chemotaxonomic Significance of Saponins from Wild and Cultured Asparagus Shoots.
Chileh-Chelh T, López-Ruiz R, García-Cervantes AM, Rodríguez-García I, Rincón-Cervera MA, Ezzaitouni M, Guil-Guerrero JL. Chileh-Chelh T, et al. Molecules. 2024 Jul 18;29(14):3367. doi: 10.3390/molecules29143367. Molecules. 2024. PMID: 39064945 Free PMC article. - Non-antibiotic feed additives production by Acremonium terricola solid-fermented Camellia oleifera meal.
Zhang P, Xiong Y, Bi L, Zhong H, Ren J, Zhou B. Zhang P, et al. Bioresour Bioprocess. 2024 Sep 28;11(1):90. doi: 10.1186/s40643-024-00808-x. Bioresour Bioprocess. 2024. PMID: 39340720 Free PMC article. - Perspectives on Saponins: Food Functionality and Applications.
Timilsena YP, Phosanam A, Stockmann R. Timilsena YP, et al. Int J Mol Sci. 2023 Aug 31;24(17):13538. doi: 10.3390/ijms241713538. Int J Mol Sci. 2023. PMID: 37686341 Free PMC article. Review.