Release of core DNA from nucleosomal core particles following (ADP-ribose)n-modification in vitro - PubMed (original) (raw)
Release of core DNA from nucleosomal core particles following (ADP-ribose)n-modification in vitro
G Mathis et al. Biochem Biophys Res Commun. 1987.
Abstract
Isolated nucleosomal core particles from rat liver chromatin were ADP-ribosylated in vitro and the consequences of this modification on intranucleosomal DNA-protein interactions were studied by retention gel analysis. A separating force of 11 fN caused the complete release of 146 bp DNA fragments from ADP-ribosylated core particles, while the DNA of unmodified core particles remained protein-associated. We conclude that posttranslational (ADP-ribose)n-modification of chromatin proteins may reduce DNA-protein interactions at the nucleosomal level of chromatin organization.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous