Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase - PubMed (original) (raw)

. 1978 Oct 19;275(5681):617-24.

doi: 10.1038/275617a0.

• PMID: 360074
• DOI: 10.1038/275617a0

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

A C Chang et al. Nature. 1978.

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.

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