Age-dependent variations of antibody avidity - PubMed (original) (raw)
- PMID: 361545
- PMCID: PMC1457364
Age-dependent variations of antibody avidity
G Doria et al. Immunology. 1978 Oct.
Abstract
Age-dependent variations of antibody avidity were studied in the C3HeB/FeJ mouse. Spleen cells from donors of different ages (10--720 days) were transferred and stimulated with TNP-HRBC in lethally irradiated syngenic recipients. The anti-TNP antibody response of the donor cells was estimated from the number of direct PFC per recipient spleen by the Jerne technique with TNP-SRBC. Avidity of the antibodies secreted by PFC was evaluated from the amount of added TNP-BSA that inhibited 50% of the anti-TNP PFC. Under these experimental conditions allowing the exclusion of any influence of the donor milieu during the immune response, age-dependent variations of the antibody response and avidity could be attributed to changes in the donor spleen cell population. Avidity was found to increase with the response and to vary parabolically with age. After appropriate correction of the number of PFC to make it independent from age, avidity values were fitted by a multiple curvilinear regression in which the independent variables playing a significant role were the corrected number of PFC in its linear term and the age in its linear and quadratic terms. From comparison of the standard coefficients of this regression, the observed variations of avidity could be attributed in part (82%) to the response and in part (18%) to the age. For any value of response, avidity increased 15-fold from day 10 to reach a maximum at day 110 and then declined 5-fold at the age of 720 days. Heterogeneity of avidity also changed parabolically with age as high avidity classes were present in adulthood and absent at 10 and 720 days.
Similar articles
- Secretion rate independent evaluation of IgM antibody avidity at the level of single immunocytes.
Doria G, Mancini C, DeLisi C. Doria G, et al. J Immunol. 1978 Nov;121(5):2030-4. J Immunol. 1978. PMID: 81861 - Macrophage requirement for induction of in vitro anti-2,4,6-trinitrophenyl response in low-affinity receptor lymphocytes.
Carvajal RE, Alanís F, Córdoba F. Carvajal RE, et al. J Reticuloendothel Soc. 1983 Jun;33(6):467-76. J Reticuloendothel Soc. 1983. PMID: 6854560
Cited by
- Type I interferon underlies severe disease associated with Junín virus infection in mice.
Hickerson BT, Sefing EJ, Bailey KW, Van Wettere AJ, Penichet ML, Gowen BB. Hickerson BT, et al. Elife. 2020 May 26;9:e55352. doi: 10.7554/eLife.55352. Elife. 2020. PMID: 32452770 Free PMC article. - Molecular composition of the alveolar lining fluid in the aging lung.
Moliva JI, Rajaram MV, Sidiki S, Sasindran SJ, Guirado E, Pan XJ, Wang SH, Ross P Jr, Lafuse WP, Schlesinger LS, Turner J, Torrelles JB. Moliva JI, et al. Age (Dordr). 2014 Jun;36(3):9633. doi: 10.1007/s11357-014-9633-4. Epub 2014 Mar 3. Age (Dordr). 2014. PMID: 24584696 Free PMC article. - Changes in immune function in asthma in the elderly.
Mathur SK, Nyenhuis SM. Mathur SK, et al. Aging health. 2009 Sep;5(4):551-559. doi: 10.2217/ahe.09.47. Aging health. 2009. PMID: 22639679 Free PMC article. - Role of allergen sensitization in older adults.
Viswanathan RK, Mathur SK. Viswanathan RK, et al. Curr Allergy Asthma Rep. 2011 Oct;11(5):427-33. doi: 10.1007/s11882-011-0204-9. Curr Allergy Asthma Rep. 2011. PMID: 21667198 Free PMC article. Review. - Allergy and asthma in the elderly.
Mathur SK. Mathur SK. Semin Respir Crit Care Med. 2010 Oct;31(5):587-95. doi: 10.1055/s-0030-1265899. Epub 2010 Oct 12. Semin Respir Crit Care Med. 2010. PMID: 20941659 Free PMC article. Review.
References
- J Immunol. 1976 Feb;116(2):279-87 - PubMed
- Cell Immunol. 1976 Feb;21(2):201-16 - PubMed
- J Exp Med. 1975 Feb 1;141(2):269-86 - PubMed
- Fed Proc. 1975 Feb;34(2):153-8 - PubMed
- J Exp Med. 1975 May 1;141(5):1133-46 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous