Hyperoxia increases H2O2 production by brain in vivo - PubMed (original) (raw)
Hyperoxia increases H2O2 production by brain in vivo
T Yusa et al. J Appl Physiol (1985). 1987 Jul.
Abstract
Hyperoxia and hyperbaric hyperoxia increased the rate of cerebral hydrogen peroxide (H2O2) production in unanesthetized rats in vivo, as measured by the H2O2-mediated inactivation of endogenous catalase activity following injection of 3-amino-1,2,4-triazole. Brain catalase activity in rats breathing air (0.2 ATA O2) decreased to 75, 61, and 40% of controls due to endogenous H2O2 production at 30, 60, and 120 min, respectively, after intraperitoneal injection of 3-amino-1,2,4-triazole. The rate of catalase inactivation increased linearly in rats exposed to 0.6 ATA O2 (3 ATA air), 1.0 ATA O2 (normobaric 100% O2) and 3.0 ATA O2 (3 ATA 100% O2) compared with 0.2 ATA O2 (room air). Catalase inactivation was prevented by pretreatment of rats with ethanol (4 g/kg), a competitive substrate for the reactive catalase-H2O2 intermediate, compound I. This confirmed that catalase inactivation by 3-amino-1,2,4-triazole was due to formation of the catalase-H2O2 intermediate, compound I. The linear rate of catalase inactivation allows estimates of the average steady-state H2O2 concentration within brain peroxisomes to be calculated from the formula: [H2O2] = 6.6 pM + 5.6 ATA-1 X pM X [O2], where [O2] is the concentration of oxygen in ATA that the rats are breathing. Thus the H2O2 concentration in brains of rats exposed to room air is calculated to be about 7.7 pM, rises 60% when O2 tension is increased to 100% O2, and increases 300% at 3 ATA 100% O2, where symptoms of central nervous system toxicity first become apparent. These studies support the concept that H2O2 is an important mediator of O2-induced injury to the central nervous system.
Similar articles
- Regional H2O2 concentration in rat brain after hyperoxic convulsions.
Piantadosi CA, Tatro LG. Piantadosi CA, et al. J Appl Physiol (1985). 1990 Nov;69(5):1761-6. doi: 10.1152/jappl.1990.69.5.1761. J Appl Physiol (1985). 1990. PMID: 2272969 - Prevention of H2O2 generation by monoamine oxidase protects against CNS O2 toxicity.
Zhang J, Piantadosi CA. Zhang J, et al. J Appl Physiol (1985). 1991 Sep;71(3):1057-61. doi: 10.1152/jappl.1991.71.3.1057. J Appl Physiol (1985). 1991. PMID: 1757301 - Ethanol-stimulated behaviour in mice is modulated by brain catalase activity and H2O2 rate of production.
Pastor R, Sanchis-Segura C, Aragon CM. Pastor R, et al. Psychopharmacology (Berl). 2002 Dec;165(1):51-9. doi: 10.1007/s00213-002-1241-9. Epub 2002 Oct 25. Psychopharmacology (Berl). 2002. PMID: 12474118 - [Catalase: structure, properties, functions].
Scibior D, Czeczot H. Scibior D, et al. Postepy Hig Med Dosw (Online). 2006;60:170-80. Postepy Hig Med Dosw (Online). 2006. PMID: 16618987 Review. Polish. - The role of hydrogen peroxide production and catalase in hepatic ethanol metabolism.
Thurman RG, Oshino N, Chance B. Thurman RG, et al. Adv Exp Med Biol. 1975;59:163-83. doi: 10.1007/978-1-4757-0632-1_13. Adv Exp Med Biol. 1975. PMID: 170803 Review. No abstract available.
Cited by
- High arterial oxygen levels and supplemental oxygen administration in traumatic brain injury: insights from CENTER-TBI and OzENTER-TBI.
Rezoagli E, Petrosino M, Rebora P, Menon DK, Mondello S, Cooper DJ, Maas AIR, Wiegers EJA, Galimberti S, Citerio G; CENTER-TBI, OzENTER-TBI Participants and Investigators. Rezoagli E, et al. Intensive Care Med. 2022 Dec;48(12):1709-1725. doi: 10.1007/s00134-022-06884-x. Epub 2022 Oct 20. Intensive Care Med. 2022. PMID: 36264365 Free PMC article. - Oxygen toxicity: cellular mechanisms in normobaric hyperoxia.
Alva R, Mirza M, Baiton A, Lazuran L, Samokysh L, Bobinski A, Cowan C, Jaimon A, Obioru D, Al Makhoul T, Stuart JA. Alva R, et al. Cell Biol Toxicol. 2023 Feb;39(1):111-143. doi: 10.1007/s10565-022-09773-7. Epub 2022 Sep 16. Cell Biol Toxicol. 2023. PMID: 36112262 Free PMC article. Review. - Molecular and Therapeutic Aspects of Hyperbaric Oxygen Therapy in Neurological Conditions.
Fischer I, Barak B. Fischer I, et al. Biomolecules. 2020 Aug 27;10(9):1247. doi: 10.3390/biom10091247. Biomolecules. 2020. PMID: 32867291 Free PMC article. Review. - CNS function and dysfunction during exposure to hyperbaric oxygen in operational and clinical settings.
Ciarlone GE, Hinojo CM, Stavitzski NM, Dean JB. Ciarlone GE, et al. Redox Biol. 2019 Oct;27:101159. doi: 10.1016/j.redox.2019.101159. Epub 2019 Mar 9. Redox Biol. 2019. PMID: 30902504 Free PMC article. Review. - The effects of low-pressure hyperbaric oxygen treatment before and after maximal exercise on lactate concentration, heart rate recovery, and antioxidant capacity.
Park SH, Park SJ, Shin MS, Kim CK. Park SH, et al. J Exerc Rehabil. 2018 Dec 27;14(6):980-984. doi: 10.12965/jer.1836468.234. eCollection 2018 Dec. J Exerc Rehabil. 2018. PMID: 30656158 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials