Identification of nuclear cap specific proteins in HeLa cells - PubMed (original) (raw)
Identification of nuclear cap specific proteins in HeLa cells
F Rozen et al. Nucleic Acids Res. 1987.
Free PMC article
Abstract
Two polypeptides of apparent molecular mass of 20 and 115 kilodaltons in nuclear fractions from HeLa cells were shown to recognize and be crosslinked to the cap structure of eukaryotic mRNAs in a cap-dependent fashion. Crosslinking of the 20 and 115 kDa polypeptides was sensitive to inhibition by low concentrations of the cap analogue m7GDP and resistant to inhibition by high KCl concentrations. In addition, crosslinking of these polypeptides to the cap structure occurred in nuclear extracts prepared from poliovirus-infected cells, under conditions where cytoplasmic cap binding proteins were incapable of interacting with the mRNA cap structure. The possible function of nuclear cap binding proteins is discussed.
Similar articles
- CAP binding proteins associated with the nucleus.
Patzelt E, Blaas D, Kuechler E. Patzelt E, et al. Nucleic Acids Res. 1983 Sep 10;11(17):5821-35. doi: 10.1093/nar/11.17.5821. Nucleic Acids Res. 1983. PMID: 6889136 Free PMC article. - A nuclear cap binding protein from HeLa cells.
Ohno M, Kataoka N, Shimura Y. Ohno M, et al. Nucleic Acids Res. 1990 Dec 11;18(23):6989-95. doi: 10.1093/nar/18.23.6989. Nucleic Acids Res. 1990. PMID: 2148205 Free PMC article. - Proteins associated with rabbit reticulocyte mRNA caps during translation as investigated by photocrosslinking.
Greenberg JR, Burn VE. Greenberg JR, et al. Nucleic Acids Res. 1988 Apr 25;16(8):3437-54. doi: 10.1093/nar/16.8.3437. Nucleic Acids Res. 1988. PMID: 3131737 Free PMC article. - Probing the function of the eukaryotic 5'-cap structure using monoclonal antibodies to cap-binding proteins.
Sonenberg N, Trachsel H. Sonenberg N, et al. Curr Top Cell Regul. 1982;21:65-88. doi: 10.1016/b978-0-12-152821-8.50007-4. Curr Top Cell Regul. 1982. PMID: 6754270 Review. No abstract available. - Cap and cap-binding proteins in the control of gene expression.
Topisirovic I, Svitkin YV, Sonenberg N, Shatkin AJ. Topisirovic I, et al. Wiley Interdiscip Rev RNA. 2011 Mar-Apr;2(2):277-98. doi: 10.1002/wrna.52. Epub 2010 Oct 28. Wiley Interdiscip Rev RNA. 2011. PMID: 21957010 Review.
Cited by
- The Nuclear Cap-Binding Complex, a multitasking binding partner of RNA polymerase II transcripts.
Kataoka N. Kataoka N. J Biochem. 2023 Dec 20;175(1):9-15. doi: 10.1093/jb/mvad081. J Biochem. 2023. PMID: 37830942 Free PMC article. Review. - Strength in Diversity: Nuclear Export of Viral RNAs.
Gales JP, Kubina J, Geldreich A, Dimitrova M. Gales JP, et al. Viruses. 2020 Sep 11;12(9):1014. doi: 10.3390/v12091014. Viruses. 2020. PMID: 32932882 Free PMC article. Review. - Integration of mRNP formation and export.
Björk P, Wieslander L. Björk P, et al. Cell Mol Life Sci. 2017 Aug;74(16):2875-2897. doi: 10.1007/s00018-017-2503-3. Epub 2017 Mar 17. Cell Mol Life Sci. 2017. PMID: 28314893 Free PMC article. Review. - The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion.
Singh G, Pratt G, Yeo GW, Moore MJ. Singh G, et al. Annu Rev Biochem. 2015;84:325-54. doi: 10.1146/annurev-biochem-080111-092106. Epub 2015 Mar 11. Annu Rev Biochem. 2015. PMID: 25784054 Free PMC article. Review. - Novel and essential subunits in the 300-kilodalton nuclear cap binding complex of Trypanosoma brucei.
Li H, Tschudi C. Li H, et al. Mol Cell Biol. 2005 Mar;25(6):2216-26. doi: 10.1128/MCB.25.6.2216-2226.2005. Mol Cell Biol. 2005. PMID: 15743819 Free PMC article.
References
- J Biol Chem. 1977 Feb 25;252(4):1181-8 - PubMed
- Nature. 1977 Mar 17;266(5599):235-9 - PubMed
- Proc Natl Acad Sci U S A. 1978 Oct;75(10):4843-7 - PubMed
- Microbiol Rev. 1980 Jun;44(2):175-205 - PubMed
- Nucleic Acids Res. 1981 Apr 10;9(7):1643-56 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous