Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein - PubMed (original) (raw)

Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator protein

M A Muesing et al. Cell. 1987.

Abstract

HIV LTR-directed expression is markedly stimulated in trans by coexpression of a region of the HIV genome encoding a portion of the tat reading frame. Transient expression assay analysis reveals that trans-activation of LTR-directed expression results primarily from an increase in mRNA accumulation. Deletion analysis of the LTR indicates that upstream promoter and enhancer elements are dispensible for trans-activation, while sequences 3' of the RNA start site displaying strict orientation and position dependence are required. These sequences, contained in the 5' leader of all HIV transcripts, form a stable stem-loop structure with twofold symmetry in the cognate mRNA. Analysis of mutations in the trans-acting region demonstrates that the trans-activator is the protein product of the tat gene, identified biochemically in HIV-infected and transfected cells as an Mr 15,000 polypeptide. We discuss possible mechanisms whereby the interaction of p15tat with the dyad element promotes the accumulation of LTR-directed mRNA.

PubMed Disclaimer

Similar articles

• Transcriptional but not translational regulation of HIV-1 by the tat gene product.
  Rice AP, Mathews MB. Rice AP, et al. Nature. 1988 Apr 7;332(6164):551-3. doi: 10.1038/332551a0. Nature. 1988. PMID: 2833703
• Anti-termination of transcription within the long terminal repeat of HIV-1 by tat gene product.
  Kao SY, Calman AF, Luciw PA, Peterlin BM. Kao SY, et al. Nature. 1987 Dec 3-9;330(6147):489-93. doi: 10.1038/330489a0. Nature. 1987. PMID: 2825027
• Human immunodeficiency viral long terminal repeat is functional and can be trans-activated in Escherichia coli.
  Kashanchi F, Wood C. Kashanchi F, et al. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2157-61. doi: 10.1073/pnas.86.7.2157. Proc Natl Acad Sci U S A. 1989. PMID: 2648391 Free PMC article.
• HIV-1 tat trans-activation requires the loop sequence within tar.
  Feng S, Holland EC. Feng S, et al. Nature. 1988 Jul 14;334(6178):165-7. doi: 10.1038/334165a0. Nature. 1988. PMID: 3386755
• HIV trans-activation and transcription control mechanisms.
  Jones KA. Jones KA. New Biol. 1989 Nov;1(2):127-35. New Biol. 1989. PMID: 2562218 Review.

Cited by

• Kinetic Resolution of the Atomic 3D Structures Formed by Ground and Excited Conformational States in an RNA Dynamic Ensemble.
  Roy R, Geng A, Shi H, Merriman DK, Dethoff EA, Salmon L, Al-Hashimi HM. Roy R, et al. J Am Chem Soc. 2023 Oct 25;145(42):22964-22978. doi: 10.1021/jacs.3c04614. Epub 2023 Oct 13. J Am Chem Soc. 2023. PMID: 37831584 Free PMC article.
• The chaperone protein p32 stabilizes HIV-1 Tat and strengthens the p-TEFb/RNAPII/TAR complex promoting HIV transcription elongation.
  Li C, Mori LP, Lyu S, Bronson R, Getzler AJ, Pipkin ME, Valente ST. Li C, et al. Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2217476120. doi: 10.1073/pnas.2217476120. Epub 2022 Dec 30. Proc Natl Acad Sci U S A. 2023. PMID: 36584296 Free PMC article.
• Role of RNA Polymerase II Promoter-Proximal Pausing in Viral Transcription.
  Whelan M, Pelchat M. Whelan M, et al. Viruses. 2022 Sep 13;14(9):2029. doi: 10.3390/v14092029. Viruses. 2022. PMID: 36146833 Free PMC article. Review.
• Advances in RNA Viral Vector Technology to Reprogram Somatic Cells: The Paramyxovirus Wave.
  Sharp B, Rallabandi R, Devaux P. Sharp B, et al. Mol Diagn Ther. 2022 Jul;26(4):353-367. doi: 10.1007/s40291-022-00599-x. Epub 2022 Jun 28. Mol Diagn Ther. 2022. PMID: 35763161 Review.
• Co-crystal structures of HIV TAR RNA bound to lab-evolved proteins show key roles for arginine relevant to the design of cyclic peptide TAR inhibitors.
  Chavali SS, Mali SM, Jenkins JL, Fasan R, Wedekind JE. Chavali SS, et al. J Biol Chem. 2020 Dec 4;295(49):16470-16486. doi: 10.1074/jbc.RA120.015444. Epub 2020 Oct 13. J Biol Chem. 2020. PMID: 33051202 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • LANL HIV Databases