Variability of the turgor pressure of individual cells of the gram-negative heterotroph Ancylobacter aquaticus - PubMed (original) (raw)

Variability of the turgor pressure of individual cells of the gram-negative heterotroph Ancylobacter aquaticus

M F Pinette et al. J Bacteriol. 1987 Oct.

Abstract

Cells of Ancylobacter aquaticus were observed under phase microscopy in a chamber to which a measured pressure could be applied. The initial collapse pressure (Ca), i.e., the lowest pressure needed to collapse the most pressure-sensitive gas vesicles, was measured for 69 cells. The cells were taken from cultures in low-density balanced exponential growth, and the experiments were performed quickly so that the bacteria were in a uniform physiological state at the time of measurement. The turgor pressure, Pt, is the difference between the pressure, C, that would cause collapse of vesicles when removed from the cell and Ca. In this paper we focus on the variability of Pt from cell to cell. Part of the observed variability of Ca was due to the variability of the collapse pressure of individual vesicles (standard deviation [SD] = 90 kPa), but because there were about 100 vesicles per cell and because a change in refracted light after the fifth vesicle (approximately) collapsed probably could be detected by the human eye, the pressure would only have an SD of 18.6 kPa due to this type of sampling error. The observed SD of Pt was 42 kPa, indicating that turgor pressure did vary considerably from cell to cell. However, the turgor pressure was independent of cell size. Statistical analysis showed that Pt would decrease 6.9 kPa over a cell cycle, but with too large an SD (19.9 kPa) to be significant. This implies that the observed change in Pt over the cell cycle is not statistically significant.

PubMed Disclaimer

Similar articles

• Nephelometric determination of turgor pressure in growing gram-negative bacteria.
  Koch AL, Pinette MF. Koch AL, et al. J Bacteriol. 1987 Aug;169(8):3654-63. doi: 10.1128/jb.169.8.3654-3663.1987. J Bacteriol. 1987. PMID: 3611024 Free PMC article.
• Turgor pressure responses of a gram-negative bacterium to antibiotic treatment, measured by collapse of gas vesicles.
  Pinette MF, Koch AL. Pinette MF, et al. J Bacteriol. 1988 Mar;170(3):1129-36. doi: 10.1128/jb.170.3.1129-1136.1988. J Bacteriol. 1988. PMID: 3343215 Free PMC article.
• Digital recordings of gas-vesicle collapse used to measure turgor pressure and cell-water relations of cyanobacterial cells.
  Holland DP, Walsby AE. Holland DP, et al. J Microbiol Methods. 2009 May;77(2):214-24. doi: 10.1016/j.mimet.2009.02.005. Epub 2009 Feb 20. J Microbiol Methods. 2009. PMID: 19230840
• Regulation of compatible solute accumulation in bacteria.
  Poolman B, Glaasker E. Poolman B, et al. Mol Microbiol. 1998 Jul;29(2):397-407. doi: 10.1046/j.1365-2958.1998.00875.x. Mol Microbiol. 1998. PMID: 9720860 Review.
• Regulation of microbial growth by turgor pressure.
  Rojas ER, Huang KC. Rojas ER, et al. Curr Opin Microbiol. 2018 Apr;42:62-70. doi: 10.1016/j.mib.2017.10.015. Epub 2017 Nov 7. Curr Opin Microbiol. 2018. PMID: 29125939 Review.

Cited by

• Bacterial wall as target for attack: past, present, and future research.
  Koch AL. Koch AL. Clin Microbiol Rev. 2003 Oct;16(4):673-87. doi: 10.1128/CMR.16.4.673-687.2003. Clin Microbiol Rev. 2003. PMID: 14557293 Free PMC article. Review.
• Autolysis control hypotheses for tolerance to wall antibiotics.
  Koch AL. Koch AL. Antimicrob Agents Chemother. 2001 Oct;45(10):2671-5. doi: 10.1128/AAC.45.10.2671-2675.2001. Antimicrob Agents Chemother. 2001. PMID: 11557453 Free PMC article. No abstract available.
• Biophysics of bacterial walls viewed as stress-bearing fabric.
  Koch AL. Koch AL. Microbiol Rev. 1988 Sep;52(3):337-53. doi: 10.1128/mr.52.3.337-353.1988. Microbiol Rev. 1988. PMID: 3054466 Free PMC article. Review. No abstract available.
• Staphylococcal cell wall: morphogenesis and fatal variations in the presence of penicillin.
  Giesbrecht P, Kersten T, Maidhof H, Wecke J. Giesbrecht P, et al. Microbiol Mol Biol Rev. 1998 Dec;62(4):1371-414. doi: 10.1128/MMBR.62.4.1371-1414.1998. Microbiol Mol Biol Rev. 1998. PMID: 9841676 Free PMC article. Review.
• Deformations in the cytoplasmic membrane of Escherichia coli direct the synthesis of peptidoglycan. The hernia model.
  Norris V, Manners B. Norris V, et al. Biophys J. 1993 Jun;64(6):1691-700. doi: 10.1016/S0006-3495(93)81541-8. Biophys J. 1993. PMID: 8369402 Free PMC article.

References

1. 1. J Bacteriol. 1969 Nov;100(2):1049-61 - PubMed
2. 1. J Bacteriol. 1971 Oct;108(1):236-40 - PubMed
3. 1. Arch Mikrobiol. 1971;79(4):327-37 - PubMed
4. 1. Bacteriol Rev. 1972 Mar;36(1):1-32 - PubMed
5. 1. J Bacteriol. 1975 Jun;122(3):1301-9 - PubMed

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central