Amount of RNA secondary structure required to induce an alternative splice - PubMed (original) (raw)
Amount of RNA secondary structure required to induce an alternative splice
D Solnick et al. Mol Cell Biol. 1987 Sep.
Abstract
We set up an alternative splicing system in vitro in which the relative amounts of two spliced RNAs, one containing and the other lacking a particular exon, were directly proportional to the length of an inverted repeat inserted into the flanking introns. We then used the system to measure the effect of intramolecular complementarity on alternative splicing in vivo. We found that an alternative splice was induced in vivo only when the introns contained more than approximately 50 nucleotides of perfect complementarity, that is, only when the secondary structure was much more stable than most if not all possible secondary structures in natural mRNA precursors. We showed further that intron insertions containing long complements to splice sites and a branch point inhibited splicing in vitro but not in vivo. These results raise the possibility that in cells most pre-mRNA secondary structures either are not maintained long enough to influence splicing choices, or never form at all.
Similar articles
- Alternative splicing caused by RNA secondary structure.
Solnick D. Solnick D. Cell. 1985 Dec;43(3 Pt 2):667-76. doi: 10.1016/0092-8674(85)90239-9. Cell. 1985. PMID: 4075405 - Trans splicing of mRNA precursors in vitro.
Konarska MM, Padgett RA, Sharp PA. Konarska MM, et al. Cell. 1985 Aug;42(1):165-71. doi: 10.1016/s0092-8674(85)80112-4. Cell. 1985. PMID: 3848348 - In vitro splicing of influenza viral NS1 mRNA and NS1-beta-globin chimeras: possible mechanisms for the control of viral mRNA splicing.
Plotch SJ, Krug RM. Plotch SJ, et al. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5444-8. doi: 10.1073/pnas.83.15.5444. Proc Natl Acad Sci U S A. 1986. PMID: 3461442 Free PMC article. - Secondary structure of splice sites in adenovirus mRNA precursors.
Munroe SH. Munroe SH. Nucleic Acids Res. 1984 Nov 26;12(22):8437-56. doi: 10.1093/nar/12.22.8437. Nucleic Acids Res. 1984. PMID: 6095200 Free PMC article. - Cancer-Associated Perturbations in Alternative Pre-messenger RNA Splicing.
Shkreta L, Bell B, Revil T, Venables JP, Prinos P, Elela SA, Chabot B. Shkreta L, et al. Cancer Treat Res. 2013;158:41-94. doi: 10.1007/978-3-642-31659-3_3. Cancer Treat Res. 2013. PMID: 24222354 Review.
Cited by
- Native functions of short tandem repeats.
Wright SE, Todd PK. Wright SE, et al. Elife. 2023 Mar 20;12:e84043. doi: 10.7554/eLife.84043. Elife. 2023. PMID: 36940239 Free PMC article. - HNRNPM controls circRNA biogenesis and splicing fidelity to sustain cancer cell fitness.
Ho JS, Di Tullio F, Schwarz M, Low D, Incarnato D, Gay F, Tabaglio T, Zhang J, Wollmann H, Chen L, An O, Chan THM, Hall Hickman A, Zheng S, Roudko V, Chen S, Karz A, Ahmed M, He HH, Greenbaum BD, Oliviero S, Serresi M, Gargiulo G, Mann KM, Hernando E, Mulholland D, Marazzi I, Wee DKB, Guccione E. Ho JS, et al. Elife. 2021 Jun 2;10:e59654. doi: 10.7554/eLife.59654. Elife. 2021. PMID: 34075878 Free PMC article. - RNA-Targeting Splicing Modifiers: Drug Development and Screening Assays.
Tang Z, Zhao J, Pearson ZJ, Boskovic ZV, Wang J. Tang Z, et al. Molecules. 2021 Apr 14;26(8):2263. doi: 10.3390/molecules26082263. Molecules. 2021. PMID: 33919699 Free PMC article. Review. - Conserved long-range base pairings are associated with pre-mRNA processing of human genes.
Kalmykova S, Kalinina M, Denisov S, Mironov A, Skvortsov D, Guigó R, Pervouchine D. Kalmykova S, et al. Nat Commun. 2021 Apr 16;12(1):2300. doi: 10.1038/s41467-021-22549-7. Nat Commun. 2021. PMID: 33863890 Free PMC article. - RNA structure replaces the need for U2AF2 in splicing.
Lin CL, Taggart AJ, Lim KH, Cygan KJ, Ferraris L, Creton R, Huang YT, Fairbrother WG. Lin CL, et al. Genome Res. 2016 Jan;26(1):12-23. doi: 10.1101/gr.181008.114. Epub 2015 Nov 13. Genome Res. 2016. PMID: 26566657 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 - PubMed
- Proteins. 1986 Nov;1(3):195-210 - PubMed
- Annu Rev Genet. 1982;16:113-34 - PubMed
- J Mol Biol. 1983 Jun 5;166(4):477-535 - PubMed
- Cell. 1983 Dec;35(2 Pt 1):433-40 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources