Synaptosomes possess an exocytotic pool of glutamate - PubMed (original) (raw)
. 1986 Jun;321(6072):772-3.
doi: 10.1038/321772a0.
- PMID: 3713864
- DOI: 10.1038/321772a0
Synaptosomes possess an exocytotic pool of glutamate
D G Nicholls et al. Nature. 1986 Jun.
Abstract
There is increasing evidence that L-glutamate is a major excitatory neurotransmitter in the central nervous system. Immunocytochemical studies indicate that glutamate within nerve terminals may be concentrated in vesicles and glutamate-accumulating vesicles have recently been isolated. Exocytotic release of glutamate from synaptosomes (isolated nerve terminals) has not been convincingly demonstrated, however, and remains highly controversial. In order to study the kinetics of release of endogenous L-glutamate from guinea pig cerebral cortical synaptosomes we have devised a continuous enzymatic assay. This has enabled us to identify a pool, equivalent to 15-20% of the total synaptosomal glutamate, which is capable of rapid Ca2+-dependent exocytotic release.
Similar articles
- Facilitatory effect of glutamate exocytosis from rat cerebrocortical nerve terminals by alpha-tocopherol, a major vitamin E component.
Yang TT, Wang SJ. Yang TT, et al. Neurochem Int. 2008 May;52(6):979-89. doi: 10.1016/j.neuint.2007.10.009. Epub 2007 Oct 18. Neurochem Int. 2008. PMID: 18037536 - Effects of high pressure on the release of excitatory amino acids by brain synaptosomes.
Gilman SC, Colton JS, Dutka AJ, Boogaard JS. Gilman SC, et al. Undersea Biomed Res. 1986 Dec;13(4):397-406. Undersea Biomed Res. 1986. PMID: 2880419 - Mechanisms of glutamate release elicited in rat cerebrocortical nerve endings by 'pathologically' elevated extraterminal K+ concentrations.
Raiteri L, Zappettini S, Milanese M, Fedele E, Raiteri M, Bonanno G. Raiteri L, et al. J Neurochem. 2007 Nov;103(3):952-61. doi: 10.1111/j.1471-4159.2007.04784.x. Epub 2007 Jul 27. J Neurochem. 2007. PMID: 17662048 - The relationship between cytoplasmic free Ca2+ and the release of glutamate from synaptosomes.
McMahon HT, Nicholls DG. McMahon HT, et al. Biochem Soc Trans. 1990 Jun;18(3):375-7. doi: 10.1042/bst0180375. Biochem Soc Trans. 1990. PMID: 2197130 Review. No abstract available. - Interrelationship between glutamate and membrane-bound ATPases in nerve cells.
Torgner I, Kvamme E. Torgner I, et al. Mol Chem Neuropathol. 1990 Jan;12(1):19-25. doi: 10.1007/BF03160054. Mol Chem Neuropathol. 1990. PMID: 1980585 Review.
Cited by
- Glutamate Release.
Hackett JT, Ueda T. Hackett JT, et al. Neurochem Res. 2015 Dec;40(12):2443-60. doi: 10.1007/s11064-015-1622-1. Epub 2015 May 27. Neurochem Res. 2015. PMID: 26012367 Review. - Inhibition of Synaptic Glutamate Exocytosis and Prevention of Glutamate Neurotoxicity by Eupatilin from Artemisia argyi in the Rat Cortex.
Lu CW, Wu CC, Chiu KM, Lee MY, Lin TY, Wang SJ. Lu CW, et al. Int J Mol Sci. 2022 Nov 2;23(21):13406. doi: 10.3390/ijms232113406. Int J Mol Sci. 2022. PMID: 36362193 Free PMC article. - Presynaptic modulation of amino acid release from synaptosomes.
Sherman AD, Hegwood TS, Baruah S, Waziri R. Sherman AD, et al. Neurochem Res. 1992 Feb;17(2):125-8. doi: 10.1007/BF00966789. Neurochem Res. 1992. PMID: 1371602 - Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography.
Fernández-Busnadiego R, Zuber B, Maurer UE, Cyrklaff M, Baumeister W, Lucic V. Fernández-Busnadiego R, et al. J Cell Biol. 2010 Jan 11;188(1):145-56. doi: 10.1083/jcb.200908082. J Cell Biol. 2010. PMID: 20065095 Free PMC article. - Mechanisms and function of dendritic exocytosis.
Kennedy MJ, Ehlers MD. Kennedy MJ, et al. Neuron. 2011 Mar 10;69(5):856-75. doi: 10.1016/j.neuron.2011.02.032. Neuron. 2011. PMID: 21382547 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous