Retrovirus-mediated transfer of the human glucocerebrosidase gene to Gaucher fibroblasts - PubMed (original) (raw)
- PMID: 3736391
Retrovirus-mediated transfer of the human glucocerebrosidase gene to Gaucher fibroblasts
P V Choudary et al. Mol Biol Med. 1986 Jun.
Abstract
A recombinant retrovirus carrying complementary DNA containing the complete coding sequence for human glucocerebrosidase was constructed and used to infect mouse cells and human fibroblasts from a type 2 Gaucher patient. The cDNA stably integrated in the genome of the recipient cell. Infected cells made glucocerebrosidase-cross-reacting material, and the protein was glycosylated and contained the epitope(s) missing in the type 2 mutant glucocerebrosidase. This study demonstrates the feasibility of efficiently transferring the gene encoding glucocerebrosidase to Gaucher cells using retrovirus as vector.
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