Methylation of the Hprt gene on the inactive X occurs after chromosome inactivation - PubMed (original) (raw)
Methylation of the Hprt gene on the inactive X occurs after chromosome inactivation
L F Lock et al. Cell. 1987.
Abstract
DNA sequences have previously been identified in the first intron of the mouse Hprt gene that are methylated on the inactive but not the active X chromosome. The temporal relationship between methylation of these sequences and X-inactivation was studied in teratocarcinoma cells and postimplantation mouse embryos: the sequences are unmethylated prior to X-inactivation and do not become methylated on the inactive X in most fetal cells until several days postinactivation. Such inactive X-specific methylation occurs in a significantly smaller proportion of the cells in the extra-embryonic tissues, yolk sac mesoderm and endoderm, than in the fetus. These data suggest that the inactive X-specific methylation of sequences such as those in the first intron of the Hprt gene does not play any role in the primary events of X-inactivation, but may function as part of a secondary, tissue-specific mechanism for maintaining the inactive state.
Similar articles
- Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice.
Kratzer PG, Chapman VM, Lambert H, Evans RE, Liskay RM. Kratzer PG, et al. Cell. 1983 May;33(1):37-42. doi: 10.1016/0092-8674(83)90332-x. Cell. 1983. PMID: 6678611 - CpG island promoter region methylation patterns of the inactive-X-chromosome hypoxanthine phosphoribosyltransferase (Hprt) gene.
Park JG, Chapman VM. Park JG, et al. Mol Cell Biol. 1994 Dec;14(12):7975-83. doi: 10.1128/mcb.14.12.7975-7983.1994. Mol Cell Biol. 1994. PMID: 7969137 Free PMC article. - Methylation of the hypoxanthine phosphoribosyltransferase locus on the human X chromosome: implications for X-chromosome inactivation.
Wolf SF, Jolly DJ, Lunnen KD, Friedmann T, Migeon BR. Wolf SF, et al. Proc Natl Acad Sci U S A. 1984 May;81(9):2806-10. doi: 10.1073/pnas.81.9.2806. Proc Natl Acad Sci U S A. 1984. PMID: 6585829 Free PMC article. - The X chromosome in development in mouse and man.
Monk M. Monk M. J Inherit Metab Dis. 1992;15(4):499-513. doi: 10.1007/BF01799608. J Inherit Metab Dis. 1992. PMID: 1528010 Review. - X-linked gene expression and X-chromosome inactivation: marsupials, mouse, and man compared.
VandeBerg JL, Robinson ES, Samollow PB, Johnston PG. VandeBerg JL, et al. Isozymes Curr Top Biol Med Res. 1987;15:225-53. Isozymes Curr Top Biol Med Res. 1987. PMID: 3298156 Review.
Cited by
- Functions of DNA methylation: islands, start sites, gene bodies and beyond.
Jones PA. Jones PA. Nat Rev Genet. 2012 May 29;13(7):484-92. doi: 10.1038/nrg3230. Nat Rev Genet. 2012. PMID: 22641018 Review. - Perturbed maintenance of transcriptional repression on the inactive X-chromosome in the mouse brain after Xist deletion.
Adrianse RL, Smith K, Gatbonton-Schwager T, Sripathy SP, Lao U, Foss EJ, Boers RG, Boers JB, Gribnau J, Bedalov A. Adrianse RL, et al. Epigenetics Chromatin. 2018 Aug 31;11(1):50. doi: 10.1186/s13072-018-0219-8. Epigenetics Chromatin. 2018. PMID: 30170615 Free PMC article. - Distribution of 5-methylcytosine-rich regions in the metaphase chromosomes of Vicia faba.
Frediani M, Giraldi E, Castiglione MR. Frediani M, et al. Chromosome Res. 1996 Feb;4(2):141-6. doi: 10.1007/BF02259707. Chromosome Res. 1996. PMID: 8785608 - Regulation of X-chromosome inactivation in development in mice and humans.
Goto T, Monk M. Goto T, et al. Microbiol Mol Biol Rev. 1998 Jun;62(2):362-78. doi: 10.1128/MMBR.62.2.362-378.1998. Microbiol Mol Biol Rev. 1998. PMID: 9618446 Free PMC article. Review. - Regulation of the transcriptional program by DNA methylation during human αβ T-cell development.
Rodriguez RM, Suarez-Alvarez B, Mosén-Ansorena D, García-Peydró M, Fuentes P, García-León MJ, Gonzalez-Lahera A, Macias-Camara N, Toribio ML, Aransay AM, Lopez-Larrea C. Rodriguez RM, et al. Nucleic Acids Res. 2015 Jan;43(2):760-74. doi: 10.1093/nar/gku1340. Epub 2014 Dec 24. Nucleic Acids Res. 2015. PMID: 25539926 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous