Differential efficiencies of in vitro translation of mouse c-myc transcripts differing in the 5' untranslated region - PubMed (original) (raw)
Comparative Study
Differential efficiencies of in vitro translation of mouse c-myc transcripts differing in the 5' untranslated region
A Darveau et al. Proc Natl Acad Sci U S A. 1985 Apr.
Abstract
We have studied the in vitro translational efficiencies of two murine c-myc transcripts synthesized in vitro that differ in the lengths of their 5' noncoding regions (448 and 83 nucleotides) and also in their 3' noncoding regions. When translated in a reticulocyte translation system, the shorter transcript was translated 10-fold more efficiently. These results are consistent with the hypothesis of Saito et al. [Saito, H., Hayday, A. C., Wiman, K., Hayward, W. S. & Tonegawa, S. (1983) Proc. Natl. Acad. Sci. USA 80, 7476-7480] that translation of full-length human c-myc mRNA is normally repressed, whereas in several Burkitt lymphomas that have deletions of the mRNA 5' noncoding region (resulting from translocation of the c-myc gene), translation of the c-myc mRNA is more efficient. Our results suggest that activation of murine c-myc expression by production of a more efficient mRNA might in some cases play a role in neoplastic transformation.
Similar articles
- Regulation of the human c-myc gene: 5' noncoding sequences do not affect translation.
Butnick NZ, Miyamoto C, Chizzonite R, Cullen BR, Ju G, Skalka AM. Butnick NZ, et al. Mol Cell Biol. 1985 Nov;5(11):3009-16. doi: 10.1128/mcb.5.11.3009-3016.1985. Mol Cell Biol. 1985. PMID: 3018494 Free PMC article. - cis-acting translational effects of the 5' noncoding region of c-myc mRNA.
Parkin N, Darveau A, Nicholson R, Sonenberg N. Parkin N, et al. Mol Cell Biol. 1988 Jul;8(7):2875-83. doi: 10.1128/mcb.8.7.2875-2883.1988. Mol Cell Biol. 1988. PMID: 3043198 Free PMC article. - Developmental regulation of translation by the 5' noncoding region of murine c-myc mRNA in Xenopus laevis.
Lazarus P, Parkin N, Sonenberg N. Lazarus P, et al. Oncogene. 1988 Nov;3(5):517-21. Oncogene. 1988. PMID: 3078957 - Myc family of cellular oncogenes.
DePinho R, Mitsock L, Hatton K, Ferrier P, Zimmerman K, Legouy E, Tesfaye A, Collum R, Yancopoulos G, Nisen P, et al. DePinho R, et al. J Cell Biochem. 1987 Apr;33(4):257-66. doi: 10.1002/jcb.240330404. J Cell Biochem. 1987. PMID: 3034933 Review. - [Aberrant regulation of mRNA 3' untranslated region in cancers and inflammation].
Nguyen-Chi M, Morello D. Nguyen-Chi M, et al. Med Sci (Paris). 2008 Mar;24(3):290-6. doi: 10.1051/medsci/2008243290. Med Sci (Paris). 2008. PMID: 18334178 Review. French.
Cited by
- Translational Control in Cancer.
Robichaud N, Sonenberg N, Ruggero D, Schneider RJ. Robichaud N, et al. Cold Spring Harb Perspect Biol. 2019 Jul 1;11(7):a032896. doi: 10.1101/cshperspect.a032896. Cold Spring Harb Perspect Biol. 2019. PMID: 29959193 Free PMC article. Review. - Targeted polymeric nanoparticles for cancer gene therapy.
Kim J, Wilson DR, Zamboni CG, Green JJ. Kim J, et al. J Drug Target. 2015;23(7-8):627-41. doi: 10.3109/1061186X.2015.1048519. Epub 2015 Jun 10. J Drug Target. 2015. PMID: 26061296 Free PMC article. Review. - The biological and therapeutic relevance of mRNA translation in cancer.
Blagden SP, Willis AE. Blagden SP, et al. Nat Rev Clin Oncol. 2011 May;8(5):280-91. doi: 10.1038/nrclinonc.2011.16. Epub 2011 Mar 1. Nat Rev Clin Oncol. 2011. PMID: 21364523 Review. - Structural and functional analysis of the human CD45 gene (PTPRC) upstream region: evidence for a functional promoter within the first intron of the gene.
Timón M, Beverley PC. Timón M, et al. Immunology. 2001 Feb;102(2):180-9. doi: 10.1046/j.1365-2567.2001.01177.x. Immunology. 2001. PMID: 11260323 Free PMC article. - The conserved 5'-untranslated leader of Spi-1 (PU.1) mRNA is highly structured and potently inhibits translation in vitro but not in vivo.
Hensold JO, Stratton CA, Barth D. Hensold JO, et al. Nucleic Acids Res. 1997 Jul 15;25(14):2869-76. doi: 10.1093/nar/25.14.2869. Nucleic Acids Res. 1997. PMID: 9207037 Free PMC article.
References
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- J Mol Biol. 1974 Sep 5;88(1):233-45 - PubMed
- Anal Biochem. 1976 Jan;70(1):75-85 - PubMed
- Eur J Biochem. 1976 AUG 1;67(1):247-56 - PubMed
- Nucleic Acids Res. 1979 Jan;6(1):153-66 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources