Enzyme-catalyzed processes in organic solvents - PubMed (original) (raw)

Enzyme-catalyzed processes in organic solvents

A Zaks et al. Proc Natl Acad Sci U S A. 1985 May.

Abstract

Three different lipases (porcine pancreatic, yeast, and mold) can vigorously act as catalysts in a number of nearly anhydrous organic solvents. Various transesterification reactions catalyzed by porcine pancreatic lipase in hexane obey Michaelis-Menten kinetics. The dependence of the catalytic activity of the enzyme in organic media on the pH of the aqueous solution from which it was recovered is bell-shaped, with the maximum coinciding with the pH optimum of the enzymatic activity in water. The catalytic power exhibited by the lipases in organic solvents is comparable to that displayed in water. In addition to transesterification, lipases can catalyze several other processes in organic media including esterification, aminolysis, acyl exchange, thiotransesterification, and oximolysis; some of these reactions proceed to an appreciable extent only in nonaqueous solvents.

PubMed Disclaimer

References

    1. Arch Biochem Biophys. 1966 Jul;115(1):44-7 - PubMed
    1. Science. 1983 Feb 11;219(4585):722-7 - PubMed
    1. Science. 1984 Jun 15;224(4654):1249-51 - PubMed
    1. Biochem Biophys Res Commun. 1984 Jul 31;122(2):845-50 - PubMed
    1. Adv Protein Chem. 1959;14:1-63 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources