Cap-dependent RNA splicing in a HeLa nuclear extract - PubMed (original) (raw)
Cap-dependent RNA splicing in a HeLa nuclear extract
I Edery et al. Proc Natl Acad Sci U S A. 1985 Nov.
Abstract
We have studied the involvement of the 5' cap structure in the splicing of precursor mRNAs in a HeLa nuclear extract. We show that precursor mRNAs are spliced efficiently only when they possess a cap structure and that preincubation of a HeLa nuclear extract rendered the splicing reaction highly sensitive to inhibition by cap analogues. This sensitization was dependent on exogenous Mg2+ but not exogenous ATP or GTP. These results demonstrate that splicing in a nuclear extract is highly dependent on the cap structure, as was demonstrated for the splicing process in a HeLa whole-cell extract [Konarska, M. M., Padget, R. A. & Sharp, P. A. (1984) Cell 38, 731-736], and thus support the contention that cap recognition is an important feature of eukaryotic mRNA biogenesis.
Similar articles
- Preferential excision of the 5' proximal intron from mRNA precursors with two introns as mediated by the cap structure.
Ohno M, Sakamoto H, Shimura Y. Ohno M, et al. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5187-91. doi: 10.1073/pnas.84.15.5187. Proc Natl Acad Sci U S A. 1987. PMID: 2440046 Free PMC article. - Recognition of cap structure in splicing in vitro of mRNA precursors.
Konarska MM, Padgett RA, Sharp PA. Konarska MM, et al. Cell. 1984 Oct;38(3):731-6. doi: 10.1016/0092-8674(84)90268-x. Cell. 1984. PMID: 6567484 - Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei.
Inoue K, Ohno M, Sakamoto H, Shimura Y. Inoue K, et al. Genes Dev. 1989 Sep;3(9):1472-9. doi: 10.1101/gad.3.9.1472. Genes Dev. 1989. PMID: 2606355 - Potential therapeutic applications of RNA cap analogs.
Ziemniak M, Strenkowska M, Kowalska J, Jemielity J. Ziemniak M, et al. Future Med Chem. 2013 Jun;5(10):1141-72. doi: 10.4155/fmc.13.96. Future Med Chem. 2013. PMID: 23795970 Review. - 5'-Terminal caps, cap-binding proteins and eukaryotic mRNA function.
Shatkin AJ, Darzynkiewicz E, Furuichi Y, Kroath H, Morgan MA, Tahara SM, Yamakawa M. Shatkin AJ, et al. Biochem Soc Symp. 1982;47:129-43. Biochem Soc Symp. 1982. PMID: 6765492 Review. No abstract available.
Cited by
- CAPturAM, a Chemo-Enzymatic Strategy for Selective Enrichment and Detection of Physiological CAPAM-Targets.
Muthmann N, Albers M, Rentmeister A. Muthmann N, et al. Angew Chem Int Ed Engl. 2023 Jan 23;62(4):e202211957. doi: 10.1002/anie.202211957. Epub 2022 Dec 19. Angew Chem Int Ed Engl. 2023. PMID: 36282111 Free PMC article. - Eukaryotic mRNA decapping factors: molecular mechanisms and activity.
He F, Jacobson A. He F, et al. FEBS J. 2023 Nov;290(21):5057-5085. doi: 10.1111/febs.16626. Epub 2022 Sep 30. FEBS J. 2023. PMID: 36098474 Free PMC article. Review. - Quantification of mRNA cap-modifications by means of LC-QqQ-MS.
Muthmann N, Špaček P, Reichert D, van Dülmen M, Rentmeister A. Muthmann N, et al. Methods. 2022 Jul;203:196-206. doi: 10.1016/j.ymeth.2021.05.018. Epub 2021 May 28. Methods. 2022. PMID: 34058305 Free PMC article. - Practical Synthesis of Cap-4 RNA.
Leiter J, Reichert D, Rentmeister A, Micura R. Leiter J, et al. Chembiochem. 2020 Jan 15;21(1-2):265-271. doi: 10.1002/cbic.201900590. Epub 2019 Nov 8. Chembiochem. 2020. PMID: 31626389 Free PMC article. - Upregulation of NPL4 promotes bladder cancer cell proliferation by inhibiting DXO destabilization of cyclin D1 mRNA.
Lu BS, Yin YW, Zhang YP, Guo PY, Li W, Liu KL. Lu BS, et al. Cancer Cell Int. 2019 May 30;19:149. doi: 10.1186/s12935-019-0874-2. eCollection 2019. Cancer Cell Int. 2019. PMID: 31164795 Free PMC article.
References
- J Mol Biol. 1977 Jan 15;109(2):173-83 - PubMed
- Nucleic Acids Res. 1983 Mar 11;11(5):1337-48 - PubMed
- Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 - PubMed
- Proc Natl Acad Sci U S A. 1983 Sep;80(17):5230-4 - PubMed
- Cell. 1983 Nov;35(1):89-99 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous