V-myc- and c-myc-encoded proteins are associated with the nuclear matrix - PubMed (original) (raw)

V-myc- and c-myc-encoded proteins are associated with the nuclear matrix

R N Eisenman et al. Mol Cell Biol. 1985 Jan.

Abstract

A series of extraction procedures were applied to avian nuclei which allowed us to define three types of association of v-myc- and c-myc-encoded proteins with nuclei: (i) a major fraction (60 to 90%) which is retained in DNA- and RNA-depleted nuclei after low- and high-salt extraction, (ii) a small fraction (1%) released during nuclease digestion of DNA in intact nuclei in the presence of low-salt buffer, and (iii) a fraction of myc protein (less than 10%) extractable with salt or detergents and found to have affinity for both single- and double-stranded DNA. Immunofluorescence analysis with anti-myc peptide sera on cells extracted sequentially with nucleases and salts confirmed the idea that myc proteins were associated with a complex residual nuclear structure (matrix-lamin fraction) which also contained avian nuclear lamin protein. Dispersal of myc proteins into the cytoplasm was found to occur during mitosis. Both c-myc and v-myc proteins were associated with the matrix-lamin, suggesting that the function of myc may relate to nuclear structural organization.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Biol Chem. 1981 Nov 25;256(22):11854-8 - PubMed
    1. EMBO J. 1983;2(12):2385-9 - PubMed
    1. Nature. 1980 Nov 13;288(5787):170-2 - PubMed
    1. Cell. 1984 Feb;36(2):241-7 - PubMed
    1. Avian Pathol. 1980 Jul;9(3):311-29 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources