Progenitors for Ly-1 B cells are distinct from progenitors for other B cells - PubMed (original) (raw)
Comparative Study
Progenitors for Ly-1 B cells are distinct from progenitors for other B cells
K Hayakawa et al. J Exp Med. 1985.
Abstract
Data from previous multiparameter fluorescence-activated cell sorter (FACS) analysis and sorting studies define a subset of murine B cells that expresses the Ly-1 surface determinant in conjunction with IgM, IgD, Ia, and other typical B cell markers. These Ly-1 B cells are physically and functionally distinct. They express more IgM and less IgD than most other B cells; they are not normally found in lymph node or bone marrow; they are always present at low frequencies (1-5%) in normal spleens, and, as we show here, they comprise about half of the B cells (10-20% of total cells) recovered from the peritoneal cavity in normal mice. Furthermore, most of the commonly studied IgM autoantibodies in normal and autoimmune mice are produced by these Ly-1 B cells, even though they seldom produce antibodies to exogenous antigens such as trinitrophenyl-Ficoll or trinitrophenyl-keyhole limpet hemocyanin. Cell transfer studies presented here demonstrate that the progenitors of Ly-1 B cells are different from the progenitors of the predominant B cell populations in spleen and lymph node. In these studies, we used FACS analysis and functional assays to characterize donor-derived (allotype-marked) B cells present in lethally irradiated recipients 1-2 mo after transfer. Surprisingly, adult bone marrow cells typically used to reconstitute B cells in irradiated recipients selectively failed to reconstitute the Ly-1 B subset. Liver, spleen, and bone marrow cells from young mice, in contrast, reconstituted all B cells (including Ly-1 B), and peritoneal "washout" cells (PerC) from adult mice uniquely reconstituted Ly-1 B. Bone marrow did not block Ly-1 B development, since PerC and newborn liver still gave rise to Ly-1 B when jointly transferred with marrow. These findings tentatively assign Ly-1 B to a distinct developmental lineage originating from progenitors that inhabit the same locations as other B cell progenitors in young animals, but move to unique location(s) in adults.
Similar articles
- Immunoglobulin-bearing B cells reconstitute and maintain the murine Ly-1 B cell lineage.
Hayakawa K, Hardy RR, Stall AM, Herzenberg LA, Herzenberg LA. Hayakawa K, et al. Eur J Immunol. 1986 Oct;16(10):1313-6. doi: 10.1002/eji.1830161021. Eur J Immunol. 1986. PMID: 3095127 - The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.
Hayakawa K, Hardy RR, Parks DR, Herzenberg LA. Hayakawa K, et al. J Exp Med. 1983 Jan 1;157(1):202-18. doi: 10.1084/jem.157.1.202. J Exp Med. 1983. PMID: 6600267 Free PMC article. - Origin of thymic and peritoneal Ly-1 B cells.
Than S, Inaba M, Inaba K, Fukuba Y, Adachi Y, Ikehara S. Than S, et al. Eur J Immunol. 1992 May;22(5):1299-303. doi: 10.1002/eji.1830220527. Eur J Immunol. 1992. PMID: 1577068 - The CD5+ B cell: a B cell lineage with a central role in autoimmune disease?
Calvert JE, Duggan-Keen MF, Smith SW, Givan AL, Bird P. Calvert JE, et al. Autoimmunity. 1988;1(3):223-40. doi: 10.3109/08916938808997167. Autoimmunity. 1988. PMID: 2485124 Review. - Characteristics and development of the murine B-1b (Ly-1 B sister) cell population.
Stall AM, Adams S, Herzenberg LA, Kantor AB. Stall AM, et al. Ann N Y Acad Sci. 1992 May 4;651:33-43. doi: 10.1111/j.1749-6632.1992.tb24591.x. Ann N Y Acad Sci. 1992. PMID: 1376053 Review.
Cited by
- The immunology of B-1 cells: from development to aging.
Mattos MS, Vandendriessche S, Waisman A, Marques PE. Mattos MS, et al. Immun Ageing. 2024 Aug 2;21(1):54. doi: 10.1186/s12979-024-00455-y. Immun Ageing. 2024. PMID: 39095816 Free PMC article. Review. - Human VH4-34 antibodies derived from B1 cells are more frequently autoreactive than VH4-34 antibodies derived from memory cells.
Ray ME, Rothstein TL. Ray ME, et al. Front Immunol. 2023 Dec 15;14:1259827. doi: 10.3389/fimmu.2023.1259827. eCollection 2023. Front Immunol. 2023. PMID: 38162664 Free PMC article. - Sex, T Cells, and the Microbiome in Natural ABO Antibody Production in Mice.
Adam I, Motyka B, Tao K, Jeyakanthan M, Alegre ML, Cowan PJ, West LJ. Adam I, et al. Transplantation. 2023 Nov 1;107(11):2353-2363. doi: 10.1097/TP.0000000000004658. Epub 2023 Jun 8. Transplantation. 2023. PMID: 37871273 Free PMC article. - Humoral immune response and changes in peritoneal cell populations in rats immunized against two Leptospira serovars; serovar patoc and serovar pyrogenes.
Gangani D, Dissanayake W, de Silva R, Anuradha K, Karunanayake L, Fernando N, Rajapakse S, Premawansa S, Handunnetti S. Gangani D, et al. BMC Immunol. 2023 Oct 17;24(1):39. doi: 10.1186/s12865-023-00574-z. BMC Immunol. 2023. PMID: 37848809 Free PMC article. - Interferons-Implications in the Immune Response to Respiratory Viruses.
Bergeron HC, Hansen MR, Tripp RA. Bergeron HC, et al. Microorganisms. 2023 Aug 29;11(9):2179. doi: 10.3390/microorganisms11092179. Microorganisms. 2023. PMID: 37764023 Free PMC article. Review.
References
- Eur J Immunol. 1971 Jan;1(1):1-9 - PubMed
- J Immunol. 1968 Sep;101(3):446-50 - PubMed
- Immunology. 1973 Jul;25(1):33-45 - PubMed
- Cell Immunol. 1974 Jul;13(1):132-45 - PubMed
- J Exp Med. 1975 Apr 1;141(4):788-803 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical