Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes - PubMed (original) (raw)
. 1985 Aug 5;260(16):9069-72.
- PMID: 3874868
Free article
Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes
D Masson et al. J Biol Chem. 1985.
Free article
Abstract
Cytolytic granules from a T-cell line with specific cytolytic activity were isolated. Granules were solubilized and fractionated on a TSK 4000 gel filtration column. Lytic activity was eluted as a single retarded peak. Polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulfate indicated that the lytic fractions contained a single protein (perforin) with an apparent molecular weight of approximately 66 kDa. It separated well from the other proteins present in the granules. Isolated perforin polymerized and inserted into lipid bilayers in the presence of Ca2+, forming tubular structures with inner diameters varying from 6 to 16 nm. Lipid insertion of perforin was demonstrated using a membrane-restricted, photoactivatable probe. The lytic properties of perforin suggest an important role of this particular protein during cytolytic T-cell-mediated target cell lysis.
Similar articles
- Isolation and biochemical and functional characterization of perforin 1 from cytolytic T-cell granules.
Podack ER, Young JD, Cohn ZA. Podack ER, et al. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8629-33. doi: 10.1073/pnas.82.24.8629. Proc Natl Acad Sci U S A. 1985. PMID: 2417226 Free PMC article. - Cytolytic T cell granules. Isolation, structural, biochemical, and functional characterization.
Podack ER, Konigsberg PJ. Podack ER, et al. J Exp Med. 1984 Sep 1;160(3):695-710. doi: 10.1084/jem.160.3.695. J Exp Med. 1984. PMID: 6332169 Free PMC article. - Interaction of chondroitin sulfate with perforin and granzymes of cytolytic T-cells is dependent on pH.
Masson D, Peters PJ, Geuze HJ, Borst J, Tschopp J. Masson D, et al. Biochemistry. 1990 Dec 25;29(51):11229-35. doi: 10.1021/bi00503a011. Biochemistry. 1990. PMID: 2125503 - Involvement of granule proteins in T-cell-mediated cytolysis.
Krähenbühl O, Tschopp J. Krähenbühl O, et al. Nat Immun Cell Growth Regul. 1990;9(4):274-82. Nat Immun Cell Growth Regul. 1990. PMID: 2215515 Review. - Perforin-mediated target cell lysis by cytolytic T lymphocytes.
Tschopp J, Nabholz M. Tschopp J, et al. Annu Rev Immunol. 1990;8:279-302. doi: 10.1146/annurev.iy.08.040190.001431. Annu Rev Immunol. 1990. PMID: 2188665 Review. No abstract available.
Cited by
- NK cell exhaustion in the tumor microenvironment.
Jia H, Yang H, Xiong H, Luo KQ. Jia H, et al. Front Immunol. 2023 Nov 2;14:1303605. doi: 10.3389/fimmu.2023.1303605. eCollection 2023. Front Immunol. 2023. PMID: 38022646 Free PMC article. Review. - Very Unstable Genetics: How the Confluence of Microsatellite Instability and Immunotherapy Revolutionized the Treatment of Colon Cancer.
Kaunitz JD, Bejjani A. Kaunitz JD, et al. Dig Dis Sci. 2023 Sep;68(9):3494-3503. doi: 10.1007/s10620-023-08007-y. Epub 2023 Jul 5. Dig Dis Sci. 2023. PMID: 37402981 No abstract available. - Non-cytotoxic functions of CD8 T cells: "repentance of a serial killer".
Al Moussawy M, Abdelsamed HA. Al Moussawy M, et al. Front Immunol. 2022 Sep 12;13:1001129. doi: 10.3389/fimmu.2022.1001129. eCollection 2022. Front Immunol. 2022. PMID: 36172358 Free PMC article. Review. - Escaping Death: How Cancer Cells and Infected Cells Resist Cell-Mediated Cytotoxicity.
Tuomela K, Ambrose AR, Davis DM. Tuomela K, et al. Front Immunol. 2022 Mar 23;13:867098. doi: 10.3389/fimmu.2022.867098. eCollection 2022. Front Immunol. 2022. PMID: 35401556 Free PMC article. Review. - Breaching the Bacterial Envelope: The Pivotal Role of Perforin-2 (MPEG1) Within Phagocytes.
Merselis LC, Rivas ZP, Munson GP. Merselis LC, et al. Front Immunol. 2021 Feb 22;12:597951. doi: 10.3389/fimmu.2021.597951. eCollection 2021. Front Immunol. 2021. PMID: 33692780 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous