Ia-transfected L-cell fibroblasts present a lysozyme peptide but not the native protein to lysozyme-specific T cells - PubMed (original) (raw)

Ia-transfected L-cell fibroblasts present a lysozyme peptide but not the native protein to lysozyme-specific T cells

N Shastri et al. Proc Natl Acad Sci U S A. 1985 Sep.

Abstract

We studied the antigen-presenting capacity of mouse L fibroblasts transfected with genes encoding Ia polypeptides of the major histocompatibility complex (MHC). These cells function as efficient antigen-presenting cells (APC) in stimulating peptide antigen-specific MHC-restricted proliferation of long-term T-cell lines, thus establishing the capacity of Ia-expressing L-cell transfectants to present antigens to apparently normal T cells. However, in contrast to splenic APC, L-cell transfectants fail to present native hen egg-white lysozyme to the same T cells. Since this result is similar to that obtained with physiologic APC pretreated to prevent antigen degradation, it suggests that L-cell transfectants, without such pretreatments, may be compromised in their ability to process native lysozyme. However, since such transfectant cells have been shown to present other complex polypeptides such as keyhole limpet hemocyanin, a random copolymer of glutamic acid, alanine, and tyrosine, and influenza virus neuraminidase, this observation suggests that protein antigens differ in the stringency of processing requirements.

PubMed Disclaimer

Cited by

Two genetically identical antigen-presenting cell clones display heterogeneity in antigen processing.
Michalek MT, Benacerraf B, Rock KL. Michalek MT, et al. Proc Natl Acad Sci U S A. 1989 May;86(9):3316-20. doi: 10.1073/pnas.86.9.3316. Proc Natl Acad Sci U S A. 1989. PMID: 2470101 Free PMC article.
Analysis of antigen presentation using HLA transfectants.
Altmann DM, Wilkinson D, Ikeda H, Trowsdale J. Altmann DM, et al. Immunol Res. 1990;9(1):57-68. doi: 10.1007/BF02918479. Immunol Res. 1990. PMID: 2406352 Review. No abstract available.
Role of receptor-binding activity of the viral hemagglutinin molecule in the presentation of influenza virus antigens to helper T cells.
Eisenlohr LC, Gerhard W, Hackett CJ. Eisenlohr LC, et al. J Virol. 1987 May;61(5):1375-83. doi: 10.1128/JVI.61.5.1375-1383.1987. J Virol. 1987. PMID: 2952806 Free PMC article.
Cytotoxic T lymphocyte antigen-4 (CTLA-4) regulates primary and secondary peptide-specific CD4(+) T cell responses.
Chambers CA, Kuhns MS, Allison JP. Chambers CA, et al. Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8603-8. doi: 10.1073/pnas.96.15.8603. Proc Natl Acad Sci U S A. 1999. PMID: 10411922 Free PMC article.
Cotransfer of the Ed alpha and Ad beta genes into L cells results in the surface expression of a functional mixed-isotype Ia molecule.
Malissen B, Shastri N, Pierres M, Hood L. Malissen B, et al. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3958-62. doi: 10.1073/pnas.83.11.3958. Proc Natl Acad Sci U S A. 1986. PMID: 3086873 Free PMC article.

References

1. Science. 1981 Jun 12;212(4500):1229-38 - PubMed
1. J Exp Med. 1980 Oct 1;152(4):759-70 - PubMed
1. Immunogenetics. 1981 Dec;14(6):481-95 - PubMed
1. J Exp Med. 1982 Feb 1;155(2):445-59 - PubMed
1. J Immunol. 1982 May;128(5):2164-9 - PubMed

Ia-transfected L-cell fibroblasts present a lysozyme peptide but not the native protein to lysozyme-specific T cells - PubMed (original) (raw)