Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli - PubMed (original) (raw)
. 1985 Feb 10;260(3):1836-41.
- PMID: 3881443
Free article
Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli
P B Wolfe et al. J Biol Chem. 1985.
Free article
Abstract
We have examined the effects of thermosensitive mutations in secA and secY (prlA) genes on the export of proteins to the three layers of the Escherichia coli cell surface. After several hours at the nonpermissive temperature, the export of two major outer membrane proteins, lipoprotein and OmpA, is delayed, then essentially blocked, in either a secA or secY strain. These mutations also have a strong effect on the export of several proteins, such as maltose binding protein, to the periplasm, though the export of many periplasmic proteins is not affected. secA and secY block the assembly of leader peptidase, which is made without a leader sequence, into the inner membrane. However, the membrane assembly of M13 coat protein (an inner membrane protein made with an amino-terminal leader sequence) is not affected. Thus, the requirement for sec function for export does not correlate with the presence or absence of leader peptide or with a particular subcellular compartment, but rather is specific to each particular protein.
Similar articles
- Processing of preproteins by liposomes bearing leader peptidase.
Ohno-Iwashita Y, Wolfe P, Ito K, Wickner W. Ohno-Iwashita Y, et al. Biochemistry. 1984 Dec 4;23(25):6178-84. doi: 10.1021/bi00320a044. Biochemistry. 1984. PMID: 6395892 - Inhibition of purified Escherichia coli leader peptidase by the leader (signal) peptide of bacteriophage M13 procoat.
Wickner W, Moore K, Dibb N, Geissert D, Rice M. Wickner W, et al. J Bacteriol. 1987 Aug;169(8):3821-2. doi: 10.1128/jb.169.8.3821-3822.1987. J Bacteriol. 1987. PMID: 3301818 Free PMC article. - The Tsr chemosensory transducer of Escherichia coli assembles into the cytoplasmic membrane via a SecA-dependent process.
Gebert JF, Overhoff B, Manson MD, Boos W. Gebert JF, et al. J Biol Chem. 1988 Nov 15;263(32):16652-60. J Biol Chem. 1988. PMID: 2846545 - Sec-independent protein insertion into the inner E. coli membrane. A phenomenon in search of an explanation.
von Heijne G. von Heijne G. FEBS Lett. 1994 Jun 6;346(1):69-72. doi: 10.1016/0014-5793(94)00296-7. FEBS Lett. 1994. PMID: 8206161 Review. - Export of the periplasmic maltose-binding protein of Escherichia coli.
Bassford PJ Jr. Bassford PJ Jr. J Bioenerg Biomembr. 1990 Jun;22(3):401-39. doi: 10.1007/BF00763175. J Bioenerg Biomembr. 1990. PMID: 2202725 Review.
Cited by
- Roles of a Glycolipid MPIase in Sec-Independent Membrane Protein Insertion.
Nomura K, Mori S, Shimamoto K. Nomura K, et al. Membranes (Basel). 2024 Feb 8;14(2):48. doi: 10.3390/membranes14020048. Membranes (Basel). 2024. PMID: 38392675 Free PMC article. Review. - Role of a bacterial glycolipid in Sec-independent membrane protein insertion.
Nomura K, Mori S, Fujikawa K, Osawa T, Tsuda S, Yoshizawa-Kumagaye K, Masuda S, Nishio H, Yoshiya T, Yoda T, Shionyu M, Shirai T, Nishiyama KI, Shimamoto K. Nomura K, et al. Sci Rep. 2022 Jul 18;12(1):12231. doi: 10.1038/s41598-022-16304-1. Sci Rep. 2022. PMID: 35851412 Free PMC article. - Alteration of Membrane Physicochemical Properties by Two Factors for Membrane Protein Integration.
Nomura K, Yamaguchi T, Mori S, Fujikawa K, Nishiyama KI, Shimanouchi T, Tanimoto Y, Morigaki K, Shimamoto K. Nomura K, et al. Biophys J. 2019 Jul 9;117(1):99-110. doi: 10.1016/j.bpj.2019.05.014. Epub 2019 May 21. Biophys J. 2019. PMID: 31164197 Free PMC article. - SecA mediates cotranslational targeting and translocation of an inner membrane protein.
Wang S, Yang CI, Shan SO. Wang S, et al. J Cell Biol. 2017 Nov 6;216(11):3639-3653. doi: 10.1083/jcb.201704036. Epub 2017 Sep 19. J Cell Biol. 2017. PMID: 28928132 Free PMC article. - Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.
Komar J, Alvira S, Schulze RJ, Martin R, Lycklama A Nijeholt JA, Lee SC, Dafforn TR, Deckers-Hebestreit G, Berger I, Schaffitzel C, Collinson I. Komar J, et al. Biochem J. 2016 Oct 1;473(19):3341-54. doi: 10.1042/BCJ20160545. Epub 2016 Jul 19. Biochem J. 2016. PMID: 27435098 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources