Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP - PubMed (original) (raw)

• PMID: 3883192
• DOI: 10.1038/313762a0

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

D L Ollis et al. Nature. 1985.

Abstract

The 3.3-A resolution crystal structure of the large proteolytic fragment of Escherichia coli DNA polymerase I complexed with deoxythymidine monophosphate consists of two domains, the smaller of which binds zinc-deoxythymidine monophosphate. The most striking feature of the larger domain is a deep crevice of the appropriate size and shape for binding double-stranded B-DNA. A flexible subdomain may allow the enzyme to surround completely the DNA substrate, thereby allowing processive nucleotide polymerization without enzyme dissociation.

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