Interaction between RNA polymerase and a ribosomal RNA promoter of E. coli - PubMed (original) (raw)

Interaction between RNA polymerase and a ribosomal RNA promoter of E. coli

J Hamming et al. Nucleic Acids Res. 1979.

Free PMC article

Abstract

The interaction between RNA polymerase and the E. coli ribosomal (r) RNA promoter(s) of the rrnE operon has been studied by the filter-binding method. The extent of complex formation between RNA polymerase and rrnE promoter(s) is salt-dependent; ppGpp specifically inhibits interaction of RNA polymerase with the rrnE promoter(s). A tentative model is proposed for the molecular events in the early steps of rRNA initiation: a transition of the primarily formed, labile RNA polymerase-rRNA promoter complex to a more stable form is the determining step. This step is salt-sensitive; ppGpp acts on this "isomerization".

PubMed Disclaimer

References

    1. FEBS Lett. 1976 Dec 31;72(2):256-61 - PubMed
    1. Nucleic Acids Res. 1978 Jul;5(7):2321-32 - PubMed
    1. Cell. 1977 Oct;12(2):429-39 - PubMed
    1. J Mol Biol. 1978 May 15;121(2):153-77 - PubMed
    1. Eur J Biochem. 1977 Mar 15;74(1):107-13 - PubMed

MeSH terms

Substances

LinkOut - more resources