Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications - PubMed (original) (raw)

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications

H Towbin et al. Proc Natl Acad Sci U S A. 1979 Sep.

Abstract

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was obtained with no loss of resolution, but the transfer was not quantitative. The method allows detection of proteins by autoradiography and is simpler than conventional procedures. The immobilized proteins were detectable by immunological procedures. All additional binding capacity on the nitrocellulose was blocked with excess protein; then a specific antibody was bound and, finally, a second antibody directed against the first antibody. The second antibody was either radioactively labeled or conjugated to fluorescein or to peroxidase. The specific protein was then detected by either autoradiography, under UV light, or by the peroxidase reaction product, respectively. In the latter case, as little as 100 pg of protein was clearly detectable. It is anticipated that the procedure will be applicable to analysis of a wide variety of proteins with specific reactions or ligands.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Anal Biochem. 1965 Feb;10:358-61 - PubMed
    1. Biochim Biophys Acta. 1955 May;17(1):67-74 - PubMed
    1. Mol Gen Genet. 1979 Jan 16;169(1):1-6 - PubMed
    1. Arthritis Rheum. 1979 Jan;22(1):87-93 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2746-9 - PubMed

MeSH terms

Substances

LinkOut - more resources