Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer - PubMed (original) (raw)
Characterization of a "silencer" in yeast: a DNA sequence with properties opposite to those of a transcriptional enhancer
A H Brand et al. Cell. 1985 May.
Abstract
The mating type of yeast is determined by the allele, either a or alpha, at the MAT locus. Two other loci, HML and HMR, contain complete copies of the alpha and a genes, respectively, which are not expressed. The four SIR gene products are required in trans for repression of the silent loci, as are cis-acting sites on either side of HML and HMR, about 1000 bp from the mating-type promoters. We demonstrate that one of these cis-acting sequences, HMRE, is able to switch off at least two nonmating-type promoters. In common with enhancers, it is able to function in either orientation, relatively independently of its position with respect to the regulated promoter, and can act on promoters 2600 bp away. However since HMRE represses, rather than enhances, transcription we have called it a "silencer" sequence.
Similar articles
- A synthetic silencer mediates SIR-dependent functions in Saccharomyces cerevisiae.
McNally FJ, Rine J. McNally FJ, et al. Mol Cell Biol. 1991 Nov;11(11):5648-59. doi: 10.1128/mcb.11.11.5648-5659.1991. Mol Cell Biol. 1991. PMID: 1922068 Free PMC article. - A yeast silencer contains sequences that can promote autonomous plasmid replication and transcriptional activation.
Brand AH, Micklem G, Nasmyth K. Brand AH, et al. Cell. 1987 Dec 4;51(5):709-19. doi: 10.1016/0092-8674(87)90094-8. Cell. 1987. PMID: 3315230 - Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication.
Shore D, Stillman DJ, Brand AH, Nasmyth KA. Shore D, et al. EMBO J. 1987 Feb;6(2):461-7. doi: 10.1002/j.1460-2075.1987.tb04776.x. EMBO J. 1987. PMID: 15981337 Free PMC article. - Silencers, silencing, and heritable transcriptional states.
Laurenson P, Rine J. Laurenson P, et al. Microbiol Rev. 1992 Dec;56(4):543-60. doi: 10.1128/mr.56.4.543-560.1992. Microbiol Rev. 1992. PMID: 1480108 Free PMC article. Review. - A locus control region regulates yeast recombination.
Haber JE. Haber JE. Trends Genet. 1998 Aug;14(8):317-21. doi: 10.1016/s0168-9525(98)01501-7. Trends Genet. 1998. PMID: 9724964 Review.
Cited by
- KSHV genome harbors both constitutive and lytically induced enhancers.
Roy Chowdhury N, Gurevich V, Shamay M. Roy Chowdhury N, et al. J Virol. 2024 Jun 13;98(6):e0017924. doi: 10.1128/jvi.00179-24. Epub 2024 May 2. J Virol. 2024. PMID: 38695538 - Canalizing cell fate by transcriptional repression.
Lim B, Domsch K, Mall M, Lohmann I. Lim B, et al. Mol Syst Biol. 2024 Mar;20(3):144-161. doi: 10.1038/s44320-024-00014-z. Epub 2024 Feb 1. Mol Syst Biol. 2024. PMID: 38302581 Free PMC article. Review. - A screen for regeneration-associated silencer regulatory elements in zebrafish.
Ando K, Ou J, Thompson JD, Welsby J, Bangru S, Shen J, Wei X, Diao Y, Poss KD. Ando K, et al. Dev Cell. 2024 Mar 11;59(5):676-691.e5. doi: 10.1016/j.devcel.2024.01.004. Epub 2024 Jan 29. Dev Cell. 2024. PMID: 38290519 - Minimal requirements for the epigenetic inheritance of engineered silent chromatin domains.
Yuan AH, Moazed D. Yuan AH, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2318455121. doi: 10.1073/pnas.2318455121. Epub 2024 Jan 10. Proc Natl Acad Sci U S A. 2024. PMID: 38198529 Free PMC article. - Methylation-directed regulatory networks determine enhancing and silencing of mutation disease driver genes and explain inter-patient expression variation.
Edrei Y, Levy R, Kaye D, Marom A, Radlwimmer B, Hellman A. Edrei Y, et al. Genome Biol. 2023 Nov 28;24(1):264. doi: 10.1186/s13059-023-03094-6. Genome Biol. 2023. PMID: 38012713 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases