Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I) - PubMed (original) (raw)

Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I)

G Buell et al. Nucleic Acids Res. 1985.

Free PMC article

Abstract

Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene has been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage lambda and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone's synthesis were chosen in an SMC-lac z fusion assay. The amounts of SMC accumulated in E. coli were influenced by mutations at two chromosomal loci, lon and htpR.

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