Serotonergic terminals in the neural sheath of the blowfly nervous system: electron microscopical immunocytochemistry and 5,7-dihydroxytryptamine labelling - PubMed (original) (raw)
Serotonergic terminals in the neural sheath of the blowfly nervous system: electron microscopical immunocytochemistry and 5,7-dihydroxytryptamine labelling
D R Nässel et al. Neuroscience. 1985 May.
Abstract
With serotonin immunocytochemistry we have demonstrated an extensive plexus of immunoreactive varicose fibres in the neural sheath of the nervous system of the blowfly, Calliphora. These fibres are located in the neural sheath of the following regions: the maxillary-labial and labrofrontal nerves of the cerebral ganglia, the cervical connective, the dorsal surface of the thoracicoabdominal ganglia, two pairs of prothoracic nerves and the median abdominal nerve. We identified the serotonin-immunoreactive neural processes in the electron microscope by means of the peroxidase-antiperoxidase method. Immunoreactivity was seen in large granular vesicles (ca 100 nm), on membranes of smaller (ca 60 nm) and larger (ca 100 nm) agranular vesicles, along the inner surface of the axolemma, along neurotubules and outer membranes of mitochondria. By conventional electron microscopy we found numerous varicose neural processes in the neural sheath of some of the above regions. These varicosities are of at least two types. One type corresponds to the serotonin-immunoreactive profiles. A second type contains large granular vesicles (ca 200 nm) of variable electron density. 5,7-Dihydroxytryptamine injected into the head capsule labelled varicosities in the neural sheath, corresponding to the ones identified with serotonin immunocytochemistry. The electron-dense labelling was seen in flattened vesicles within these varicosities. We propose that the serotonin-immunoreactive fibers in the neural sheath constitute neurohemal regions for the release of serotonin into the circulation. The finding of another morphological type of varicose fibers in the neural sheath suggests the presence of further putative neurohormones in these regions.
Similar articles
- Ultrastructural demonstration of serotonin-immunoreactivity in the nervous system of an insect (Calliphora erythrocephala).
Nässel DR, Elekes K. Nässel DR, et al. Neurosci Lett. 1984 Jul 27;48(2):203-10. doi: 10.1016/0304-3940(84)90020-x. Neurosci Lett. 1984. PMID: 6384830 - Vasopressin- and proctolin-like immunoreactive efferent neurons in blowfly abdominal ganglia: development and ultrastructure.
Nässel DR, Holmqvist BI, Movérus BJ. Nässel DR, et al. J Comp Neurol. 1989 May 15;283(3):450-63. doi: 10.1002/cne.902830312. J Comp Neurol. 1989. PMID: 2568372 - Mapping and ultrastructure of serotonin-immunoreactive neurons in the optic lobes of three insect species.
Nässel DR, Meyer EP, Klemm N. Nässel DR, et al. J Comp Neurol. 1985 Feb 8;232(2):190-204. doi: 10.1002/cne.902320205. J Comp Neurol. 1985. PMID: 3973090 - Nerves and perisinusoidal cells in human liver.
Bioulac-Sage P, Lafon ME, Saric J, Balabaud C. Bioulac-Sage P, et al. J Hepatol. 1990 Jan;10(1):105-12. doi: 10.1016/0168-8278(90)90080-b. J Hepatol. 1990. PMID: 2407769 Review.
Cited by
- Neurotransmitters regulate rhythmic size changes amongst cells in the fly's optic lobe.
Pyza E, Meinertzhagen IA. Pyza E, et al. J Comp Physiol A. 1996 Jan;178(1):33-45. doi: 10.1007/BF00189588. J Comp Physiol A. 1996. PMID: 8568723 - CalpA, a Drosophila calpain homolog specifically expressed in a small set of nerve, midgut, and blood cells.
Theopold U, Pintér M, Daffre S, Tryselius Y, Friedrich P, Nässel DR, Hultmark D. Theopold U, et al. Mol Cell Biol. 1995 Feb;15(2):824-34. doi: 10.1128/MCB.15.2.824. Mol Cell Biol. 1995. PMID: 7823949 Free PMC article. - Targeted manipulation of serotonergic neurotransmission affects the escalation of aggression in adult male Drosophila melanogaster.
Alekseyenko OV, Lee C, Kravitz EA. Alekseyenko OV, et al. PLoS One. 2010 May 24;5(5):e10806. doi: 10.1371/journal.pone.0010806. PLoS One. 2010. PMID: 20520823 Free PMC article. - Light- and electron-microscopic immunocytochemistry of peptidergic neurons innervating thoracico-abdominal neurohaemal areas in the blowfly.
Duve H, Thorpe A, Nässel DR. Duve H, et al. Cell Tissue Res. 1988 Sep;253(3):583-95. doi: 10.1007/BF00219749. Cell Tissue Res. 1988. PMID: 3180185