Rapid serological analysis of bacterial lipopolysaccharides by electrotransfer to nitrocellulose - PubMed (original) (raw)
Rapid serological analysis of bacterial lipopolysaccharides by electrotransfer to nitrocellulose
S W Pyle et al. J Immunol Methods. 1985.
Abstract
Techniques are described for the rapid screening of proteinase K-treated bacterial lysates by electroblot and immunoenzymatic detection to assess O-specificity of antigens and antisera. Conditions are outlined which permit the use of a single polyacrylamide gel for both electrotransfer to nitrocellulose and silver staining. Immunodetection of transferred LPS bands was equally sensitive to silver stain when whole cell or O-specific antisera were used. The techniques were utilized to identify at least 4 O-serotypes among sorbitol fermenting isolates of the fish pathogen, Yersinia ruckeri. Observed variations in the electrophoretic mobilities of lipopolysaccharides from 17 field isolates of Y. ruckeri were used to accurately predict the O-serotype.
Similar articles
- Immunoenzymatic analysis by monoclonal antibodies of bacterial lipopolysaccharides after transfer to nitrocellulose.
Sidberry H, Kaufman B, Wright DC, Sadoff J. Sidberry H, et al. J Immunol Methods. 1985 Feb 11;76(2):299-305. doi: 10.1016/0022-1759(85)90307-2. J Immunol Methods. 1985. PMID: 2579158 - O-serotyping of Yersinia ruckeri with special emphasis on European isolates.
Davies RL. Davies RL. Vet Microbiol. 1990 May;22(4):299-307. doi: 10.1016/0378-1135(90)90016-o. Vet Microbiol. 1990. PMID: 1694607 - Detection of Yersinia enterocolitica O-antigens by double diffusion in agar and chemical characterization of lipopolysaccharides.
Dagestad K, Grov A, Oeding P. Dagestad K, et al. Acta Pathol Microbiol Scand B. 1981 Apr;89(2):81-6. doi: 10.1111/j.1699-0463.1981.tb00157_89b.x. Acta Pathol Microbiol Scand B. 1981. PMID: 6789611 - Structural and antigenic properties of lipopolysaccharides from serotype reference strains of Campylobacter jejuni.
Preston MA, Penner JL. Preston MA, et al. Infect Immun. 1987 Aug;55(8):1806-12. doi: 10.1128/iai.55.8.1806-1812.1987. Infect Immun. 1987. PMID: 3301678 Free PMC article. - [Chemical basis for the serologic specificity of the pseudotuberculosis microbe].
Shoshiev LN, Griboedov AV. Shoshiev LN, et al. Zh Mikrobiol Epidemiol Immunobiol. 1977 Mar;(3):26-31. Zh Mikrobiol Epidemiol Immunobiol. 1977. PMID: 67731 Review. Russian. No abstract available.
Cited by
- Salmonella serogroups C2 and C3 identified by agglutination using an immunoglobulin G3(kappa) monoclonal antibody (32-1-E3) reactive with a somatic factor 8-like polysaccharide antigen.
Duffey PS, Kani JC, Lee JO, Hill WJ, Kokka R. Duffey PS, et al. J Clin Microbiol. 1992 Dec;30(12):3050-7. doi: 10.1128/jcm.30.12.3050-3057.1992. J Clin Microbiol. 1992. PMID: 1452684 Free PMC article. - Characterization of a porin from the outer membrane of Vibrio anguillarum.
Simón M, Mathes A, Blanch A, Engelhardt H. Simón M, et al. J Bacteriol. 1996 Jul;178(14):4182-8. doi: 10.1128/jb.178.14.4182-4188.1996. J Bacteriol. 1996. PMID: 8763947 Free PMC article. - Lipopolysaccharide from nonvirulent Ara+ Burkholderia pseudomallei isolates is immunologically indistinguishable from lipopolysaccharide from virulent Ara- clinical isolates.
Anuntagool N, Intachote P, Wuthiekanun V, White NJ, Sirisinha S. Anuntagool N, et al. Clin Diagn Lab Immunol. 1998 Mar;5(2):225-9. doi: 10.1128/CDLI.5.2.225-229.1998. Clin Diagn Lab Immunol. 1998. PMID: 9521147 Free PMC article. - Electrophoretic and chemical characterization of lipopolysaccharides of Vibrio parahaemolyticus.
Han TJ, Chai TJ. Han TJ, et al. J Bacteriol. 1992 May;174(10):3140-6. doi: 10.1128/jb.174.10.3140-3146.1992. J Bacteriol. 1992. PMID: 1374376 Free PMC article. - Murine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup A.
Luk MC, Tsang RS, Ng MH. Luk MC, et al. J Clin Microbiol. 1987 Nov;25(11):2140-4. doi: 10.1128/jcm.25.11.2140-2144.1987. J Clin Microbiol. 1987. PMID: 2447120 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources